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SUMMARY: Twenty-six non-flagellate and two paralysed mutants of Proteus mirabilis strain 13 were investigated by transduction with phage 34.13. Abortive transduction divided fla mutants into eight complementation groups (flaA, B, C, D, E, H, J, K) while the mot mutants formed one group. Intracistronic and non-reciprocal complementation was found in certain complementation groups. With the exception of flaH, which could not be mapped, all fla genes were linked to each other and to the mot gene. A genetic map of fla and mot mutations was constructed. Representatives of the eight fla mutant groups did not elicit flagellar antibodies in rabbits and are probably unable to synthesize flagellin.
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