SUMMARY: By interrupting matings at different times, the sequential transfer of selected and unselected markers to recipient bacteria was followed in Under the best mating conditions employed, the earliest marker was transferred within 13 min and the latest within 98 min of impinging mating mixtures on membranes. All tested donor markers appeared in recombinants. A genetic map based on the entry times of markers indicated a single linkage group showing much similarity to that of The high frequency donor transferred markers in a clockwise direction, its origin corresponding to a point near 60 min on the map.


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