1887

Abstract

SUMMARY: is a bacterium whose life cycle involves sphere-rod-sphere morphogenesis. The molecular mechanism of such cellular differentiation is unknown. It is not clear whether this is regulated by simple metabolic controls such as feedback inhibition or whether expression of the genome is implicated. The present study involves a comparison of RNAs synthesized throughout the life cycle by using competitive RNA-DNA hybridization. The results show that there are differences in the RNAs synthesized at different times. The RNA appears to fall into two general categories, with some sequences always present and other sequences found only at certain times. Morphogenesis thus involves differential transcription of the genome.

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/content/journal/micro/10.1099/00221287-77-1-51
1973-07-01
2024-12-14
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References

  1. Aronson A. I. 1965; Characterization of mRNA in sporulating. Bacillus cereus, Journal of Molecular Biology 11:576–588
    [Google Scholar]
  2. Attardi G., Huang P. C., Kabat S. 1965; Recognition of ribosomal sites in DNA. I. Analysis of the Pseudomonas aeruginosa. coli system. Proceedings of the National Academy of Science 53:1490–1498
    [Google Scholar]
  3. Bautz E. K. F. 1967; The product of in vivo and in vitro transcription. In Molecular Genetics part II pp 222–233 Edited by Taylor J. H. New York: Academic Press;
    [Google Scholar]
  4. Bernardi G. 1969a; Chromatography of nucleic acids on hydroxyapatite. I. Chromatography of native DNA. Biochi mica et biophysica acta 174:423–435
    [Google Scholar]
  5. Bernardi G. 1969b; Chromatography of nucleic acids on hydroxyapatite. III. Chromatography of RNA and polyribonucleotides. Biochimica et biophysica acta 174:449–457
    [Google Scholar]
  6. Bonner J., Kung G., Bekhor I. 1967; A method for the hydridization of nucleic acid molecules at low temperature. Biochemistry 6:3650–3653
    [Google Scholar]
  7. Conn H. J., Dimmick I. 1947; Soil bacteria similar in morphology to Mycobacteria and Pseudomonas aeruginosa. Journal of Bacteriology 54:291–303
    [Google Scholar]
  8. Ensign J. C., Wolfe R. S. 1964; Nutritional control of morphogenesis in Pseudomonas aeruginosa. Journal of Bacteriology 87:924–932
    [Google Scholar]
  9. Ferdinandus J. A. 1969 Enzyme control of sphere-rod morphogenesis in Arthrobacter crystallopoietes Ph.D. Thesis, University of Oklahoma;
    [Google Scholar]
  10. Ferdinandus J. A., Clark J. B. 1969; Selective inhibition of bacterial enzymes by free fatty acids. Journal of Bacteriology 98:1109–1113
    [Google Scholar]
  11. Giacomoni D., Spiegelman S. 1962; Origin and biological individuality of the genetic dictionary. Science, New York 138:1328–1331
    [Google Scholar]
  12. Gillespie D. 1968; The formation and detection of DNA-RNA hybrids. In Methods in Enzymology vol 3 pp 641–668 Edited by Grossman L., Moldave K. New York: Academic Press;
    [Google Scholar]
  13. Gillespie S., Gillespie D. 1971; RNA-DNA hybridization in aqueous solutions and in solutions containing formamide. Biochemical Journal 125:481–488
    [Google Scholar]
  14. Gillespie D., Spiegleman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. Journal of Molecular Biology 12:829–842
    [Google Scholar]
  15. Holley R. W. 1963; Large-scale preparation of yeast ‘soluble’ ribonucleic acid. Biochemistry and Biophysics Research Communication 10:186–188
    [Google Scholar]
  16. Kennell D. 1968; Titration of the gene sites on DNA by DNA-RNA hybridization. II. The Escherichia coli chromosome. Journal of Molecular Biology 34:85–103
    [Google Scholar]
  17. Kennell D. 1971; Principles and practices of nucleic acid hybridization. In Progress in Nucleic Acid Research and Molecular Biology vol 11 pp 259–302 Edited by Davidson J. N., Cohn W. E. New York: Academic Press;
    [Google Scholar]
  18. Kennell D., Kotoulas A. 1968; Titration of the gene sites on DNA by DNA-RNA hybridization. I. Problems of measurement. Journal of Molecular Biology 34:71–84
    [Google Scholar]
  19. Krulwich T. A., Ensign J. C. 1968; Activity of an autolytic N-acetylmuramidase during sphere-rod morphogenesis of Pseudomonas aeruginosa. Journal of Bacteriology 96:857–859
    [Google Scholar]
  20. Krulwich T. A., Ensign J. C. 1969; Alteration of glucose metabolism of Arthrobacter crystallopoietes by compounds which induce sphere to rod morphogenesis. Journal of Bacteriology 97:526–534
    [Google Scholar]
  21. Krulwich T. A., Ensign J. C., Tipper D. J., Strominger J. L. 1964a; Sphere-rod morphogenesis in Arthrobacter crystallopoietes. I. Cell wall composition and polysaccharides of the peptidoglycan. Journal of Bacteriology 94:734–740
    [Google Scholar]
  22. Krulwich T. A., Ensign J. C., Tipper D. J., Strominger J. L. 1964b; Sphere-rod morphogenesis in Arthrobacter crystallopoietes. II. Peptides of the cell wall peptidoglycan. Journal of Bacteriology 94:741–750
    [Google Scholar]
  23. McConaughy B. L., Laird C. D., McCarthy B. J. 1969; Nucleic acid reassociation in formamide. Biochemistry 8:3289–3295
    [Google Scholar]
  24. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  25. Oda K. I., Joklik W. K. 1967; Hybridization and sedimentation studies on ‘early’ and Tate’ vaccinia messenger RNA. Journal of Molecular Biology 27:395–418
    [Google Scholar]
  26. Oishi M., Sueoka N. 1965; Location of genetic loci of rRNA on Pseudomonas aeruginosa. subtilis chromosome. Proceedings of National Academy of Sciences of the United States of America 54:483–491
    [Google Scholar]
  27. Stevenson I. L. 1961; Growth studies on Pseudomonas aeruginosa. Canadian Journal of Microbiology 7:569–575
    [Google Scholar]
  28. Teipel J., Koshland D. E. 1969; Significance of intermediary plateau regions in enzyme saturation curves. Biochemistry 8:4656–4663
    [Google Scholar]
  29. Webb R. B. 1954; A useful bacterial cell wall stain. Journal of Bacteriology 67:252–253
    [Google Scholar]
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