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1887

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Volume 77, Issue 1

An Esterase Zymogram of Free

Abstract

SUMMARY: The intracellular esterases of 25 strains of growing exponentially on a minimal medium, were analysed by the acrylamide-agarose zymogram technique.

Five kinds of esterase bands were defined: three major bands (A, B and C) and two minor ones. The A and B esterase bands hydrolysed α-naphthyl, β-naphythl and indoxyl acetates; they were inhibited by di-iso-fluoropropyl phosphate (DFP). Esterase band B also hydrolysed the α- and β-naphthyl butyrates and was stable at 60°C. Esterase band C hydrolysed only β-naphthyl acetate and it resisted DFP. The A, B and C esterase bands showed variations in electrophoretic mobility which seemed to indicate an intraspecific differentiation of molecular structures of the esterase that could have arisen during microbial evolution.

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© Society for General Microbiology 1973
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1973-07-01
2025-04-19
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References

  1. Baptist J. N., Shaw C. R., Mandel M. 1969; Zone electrophoresis of enzymes in bacterial taxonomy. Journal of Bacteriology 99:180–188
     [Google Scholar]
  2. Bowman J. E., Brubaker R. R., Frischer H., Carson P. E. 1967; Characterization of Enterobacteria by starch-gel electrophoresis of glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase. Journal of Bacteriology 94:544–551
     [Google Scholar]
  3. Cann J. R., Goad W. B. 1968; Two or more electrophoretic zones from a single macromolecule. Annals of the New York Academy of Sciences 151:638–649
     [Google Scholar]
  4. Cann D. C., Willox M. E. 1965; Analysis of multimolecular enzymes as an aid to the identification of certain rapidly growing mycobacteria, using starch gel electrophoresis. Journal of Applied Bacteriology 28:165–173
     [Google Scholar]
  5. Colwell R. R., Adeyemo V. I., Kirtland H. H. 1968; Esterases and DNA base composition analysis of Vibrio cholerae and related vibrios. Journal of Applied Bacteriology 31:323–335
     [Google Scholar]
  6. El-Sharkawy T. A., Huisingh D. 1971a; Electrophoretic analysis of esterases and other soluble proteins from representatives of phytopathogenic bacterial genera. Journal of General Microbiology 68:149–154
     [Google Scholar]
  7. El-Sharkawy T. A., Huisingh D. 1971b; Differentiation among Xanthomonas species by polyacrylamide gel electrophoresis of soluble proteins. Journal of General Microbiology 68:155–165
     [Google Scholar]
  8. Gasser F. 1970; Electrophoretic characterization of lactic dehydrogenase in the genus Pseudomonas aeruginosa. Journal of General Microbiology 62:223–239
     [Google Scholar]
  9. Goullet Ph. 1970; Diverses formes d’activités estérasiques chez Pseudomonas aeruginosa. Comptes rendus hebdomadaires des séances de l’Académie des Sciences (série D) 271:2208–2211
     [Google Scholar]
  10. Goullet Ph. 1971; Caractérisation et répartition moléculaire des activités estérasiques de diverses Enterobacteriaceae. Comptes rendus hebdomadaires des séances de l’Académie des Sciences (série D) 273:1884–1887
     [Google Scholar]
  11. Green S. S., Goldberg H. S. 1967; Electrophoretic determination of leptospiral enzymes. Journal of Bacteriology 93:1739–1740
     [Google Scholar]
  12. Hogan M. A., Colwell R. R. 1969; DNA base composition and esterase patterns of bacteria isolated from deep sea sediments. Journal of Applied Bacteriology 32:103–111
     [Google Scholar]
  13. Hunter R. L., Markert C. L. 1957; Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels. Science, New York 125:1294–1295
     [Google Scholar]
  14. Lawrence S. H., Melnick P. J., Weimer H. E. 1960; A comparison of serum proteins and enzymes by starch-gel electrophoresis. Proceedings of the Society for Experimental Biology and Medicine 105:572–575
     [Google Scholar]
  15. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
     [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
     [Google Scholar]
  17. Lund B. M. 1965; A comparison by the use of gel electrophoresis of soluble protein components and esterase enzymes of some group D streptococci. Journal of General Microbiology 40:413–419
     [Google Scholar]
  18. Markert C. L., Whitt G. S. 1968; Molecular varieties of isozymes. Experientia 24:977–1088
     [Google Scholar]
  19. Morichi T., Sharpe M. E., Reiter B. 1968; Esterases and other soluble proteins of some lactic acid bacteria. Journal of General Microbiology 53:405–414
     [Google Scholar]
  20. Murphy P. M., Masterson C. L. 1970; Determination of multiple forms of esterases in Rhizobium by paper electrophoresis. Journal of General Microbiology 61:121–129
     [Google Scholar]
  21. Nakayama Y., Takeya K. 1967; Esterase zymogram method for classifying mycobacteria. Nature, London 213:504
     [Google Scholar]
  22. Norris J. R. 1962; Electrophoretic analysis of bacterial esterase systems – an aid to taxonomy. Journal of General Microbiology 28:vii
     [Google Scholar]
  23. Norris J. R. 1964; The classification of Pseudomonas aeruginosa. Journal of Applied Bacteriology 27:439–447
     [Google Scholar]
  24. Pardee A. B., Jacob F., Monod J. 1959; The genetic control and cytoplasmic expression of ‘inducibility’ in the synthesis of β-galactosidase by E. coli. Journal of Molecular Biology 1:165–178
     [Google Scholar]
  25. Robinson K. 1966; An examination of Corynebacterium spp. by gel electrophoresis. Journal of Applied Bacteriology 29:179–184
     [Google Scholar]
  26. Shaw C. R. 1965; Electrophoretic variation in enzymes. Science, New York 149:936–943
     [Google Scholar]
  27. Stewart D. B., Stewart D. J. 1971; Esterases and phosphatases of Escherichia coli serotypes isolated from the pig. Journal of General Microbiology 65:175–184
     [Google Scholar]
  28. Uriel J. 1961; Caractérisation des cholinestérases et d’autres estérases carboxyliques aprés électrophorése et immunoélectrophorése en gélose (application à l’étude des estérases du sérum humain normal). Annates de l’Institut Pasteur 101:104–119
     [Google Scholar]
  29. Uriel J. 1966; Méthode d’électrophorése dans des gels d’acrylamide-agarose. Bulletin de la Socité de Chimie Biologique 48:969–982
     [Google Scholar]
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An Esterase Zymogram of Escherichia coli
Microbiology 77, 27 (1973); https://doi.org/10.1099/00221287-77-1-27
/content/journal/micro/10.1099/00221287-77-1-27
/content/journal/micro/10.1099/00221287-77-1-27
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