1887

Abstract

SUMMARY: The intracellular esterases of 25 strains of growing exponentially on a minimal medium, were analysed by the acrylamide-agarose zymogram technique.

Five kinds of esterase bands were defined: three major bands (A, B and C) and two minor ones. The A and B esterase bands hydrolysed α-naphthyl, β-naphythl and indoxyl acetates; they were inhibited by di-iso-fluoropropyl phosphate (DFP). Esterase band B also hydrolysed the α- and β-naphthyl butyrates and was stable at 60°C. Esterase band C hydrolysed only β-naphthyl acetate and it resisted DFP. The A, B and C esterase bands showed variations in electrophoretic mobility which seemed to indicate an intraspecific differentiation of molecular structures of the esterase that could have arisen during microbial evolution.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-77-1-27
1973-07-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/77/1/mic-77-1-27.html?itemId=/content/journal/micro/10.1099/00221287-77-1-27&mimeType=html&fmt=ahah

References

  1. Baptist J. N., Shaw C. R., Mandel M. 1969; Zone electrophoresis of enzymes in bacterial taxonomy. Journal of Bacteriology 99:180–188
    [Google Scholar]
  2. Bowman J. E., Brubaker R. R., Frischer H., Carson P. E. 1967; Characterization of Enterobacteria by starch-gel electrophoresis of glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase. Journal of Bacteriology 94:544–551
    [Google Scholar]
  3. Cann J. R., Goad W. B. 1968; Two or more electrophoretic zones from a single macromolecule. Annals of the New York Academy of Sciences 151:638–649
    [Google Scholar]
  4. Cann D. C., Willox M. E. 1965; Analysis of multimolecular enzymes as an aid to the identification of certain rapidly growing mycobacteria, using starch gel electrophoresis. Journal of Applied Bacteriology 28:165–173
    [Google Scholar]
  5. Colwell R. R., Adeyemo V. I., Kirtland H. H. 1968; Esterases and DNA base composition analysis of Vibrio cholerae and related vibrios. Journal of Applied Bacteriology 31:323–335
    [Google Scholar]
  6. El-Sharkawy T. A., Huisingh D. 1971a; Electrophoretic analysis of esterases and other soluble proteins from representatives of phytopathogenic bacterial genera. Journal of General Microbiology 68:149–154
    [Google Scholar]
  7. El-Sharkawy T. A., Huisingh D. 1971b; Differentiation among Xanthomonas species by polyacrylamide gel electrophoresis of soluble proteins. Journal of General Microbiology 68:155–165
    [Google Scholar]
  8. Gasser F. 1970; Electrophoretic characterization of lactic dehydrogenase in the genus Pseudomonas aeruginosa. Journal of General Microbiology 62:223–239
    [Google Scholar]
  9. Goullet Ph. 1970; Diverses formes d’activités estérasiques chez Pseudomonas aeruginosa. Comptes rendus hebdomadaires des séances de l’Académie des Sciences (série D) 271:2208–2211
    [Google Scholar]
  10. Goullet Ph. 1971; Caractérisation et répartition moléculaire des activités estérasiques de diverses Enterobacteriaceae. Comptes rendus hebdomadaires des séances de l’Académie des Sciences (série D) 273:1884–1887
    [Google Scholar]
  11. Green S. S., Goldberg H. S. 1967; Electrophoretic determination of leptospiral enzymes. Journal of Bacteriology 93:1739–1740
    [Google Scholar]
  12. Hogan M. A., Colwell R. R. 1969; DNA base composition and esterase patterns of bacteria isolated from deep sea sediments. Journal of Applied Bacteriology 32:103–111
    [Google Scholar]
  13. Hunter R. L., Markert C. L. 1957; Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels. Science, New York 125:1294–1295
    [Google Scholar]
  14. Lawrence S. H., Melnick P. J., Weimer H. E. 1960; A comparison of serum proteins and enzymes by starch-gel electrophoresis. Proceedings of the Society for Experimental Biology and Medicine 105:572–575
    [Google Scholar]
  15. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
    [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  17. Lund B. M. 1965; A comparison by the use of gel electrophoresis of soluble protein components and esterase enzymes of some group D streptococci. Journal of General Microbiology 40:413–419
    [Google Scholar]
  18. Markert C. L., Whitt G. S. 1968; Molecular varieties of isozymes. Experientia 24:977–1088
    [Google Scholar]
  19. Morichi T., Sharpe M. E., Reiter B. 1968; Esterases and other soluble proteins of some lactic acid bacteria. Journal of General Microbiology 53:405–414
    [Google Scholar]
  20. Murphy P. M., Masterson C. L. 1970; Determination of multiple forms of esterases in Rhizobium by paper electrophoresis. Journal of General Microbiology 61:121–129
    [Google Scholar]
  21. Nakayama Y., Takeya K. 1967; Esterase zymogram method for classifying mycobacteria. Nature, London 213:504
    [Google Scholar]
  22. Norris J. R. 1962; Electrophoretic analysis of bacterial esterase systems – an aid to taxonomy. Journal of General Microbiology 28:vii
    [Google Scholar]
  23. Norris J. R. 1964; The classification of Pseudomonas aeruginosa. Journal of Applied Bacteriology 27:439–447
    [Google Scholar]
  24. Pardee A. B., Jacob F., Monod J. 1959; The genetic control and cytoplasmic expression of ‘inducibility’ in the synthesis of β-galactosidase by E. coli. Journal of Molecular Biology 1:165–178
    [Google Scholar]
  25. Robinson K. 1966; An examination of Corynebacterium spp. by gel electrophoresis. Journal of Applied Bacteriology 29:179–184
    [Google Scholar]
  26. Shaw C. R. 1965; Electrophoretic variation in enzymes. Science, New York 149:936–943
    [Google Scholar]
  27. Stewart D. B., Stewart D. J. 1971; Esterases and phosphatases of Escherichia coli serotypes isolated from the pig. Journal of General Microbiology 65:175–184
    [Google Scholar]
  28. Uriel J. 1961; Caractérisation des cholinestérases et d’autres estérases carboxyliques aprés électrophorése et immunoélectrophorése en gélose (application à l’étude des estérases du sérum humain normal). Annates de l’Institut Pasteur 101:104–119
    [Google Scholar]
  29. Uriel J. 1966; Méthode d’électrophorése dans des gels d’acrylamide-agarose. Bulletin de la Socité de Chimie Biologique 48:969–982
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-77-1-27
Loading
/content/journal/micro/10.1099/00221287-77-1-27
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error