RT Journal Article SR Electronic(1) A1 Hopwood, D. A. A1 Wright, Helen M.YR 1973 T1 Transfer of a Plasmid between Streptomyces Species JF Microbiology, VO 77 IS 1 SP 187 OP 195 DO https://doi.org/10.1099/00221287-77-1-187 PB Microbiology Society, SN 1465-2080, AB SUMMARY: It has been shown previously that IF strains of Streptomyces coelicolor harbour a plasmid, SCP 1, lacking in UF strains, and that IF strains inhibit aerial mycelium production of UF strains. In the present study, IF strains of S. coelicolor, but not UF strains, were found to inhibit 19 out of 32 wild-type streptomycetes belonging to various species. Some of these presumptive UF wild-types were grown in mixed culture with IF strains of A3(2) and it was found, by a simple visual procedure, that SCP 1 was transferred to one of them, converting it to an IF strain. The rates of loss of SCP 1 from this strain, both spontaneously and after u.v. irradiation, were found to be similar to those from IF strains of A3(2). The plasmid had no detectable effect on recombination within the new strain., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-77-1-187