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Abstract
SUMMARY: A circular map of two strains of Streptomyces rimosus was constructed by the analysis of several hundred heteroclones. Segregants from four-point and five-point heteroclones were scored and the markers arranged by the criterion of minimizing the number of quadruple crossovers. Distances between genes were estimated from the analysis of the segregation of pairs of loci, whenever the ratio between the frequency of parental segregants approached the ratio between those of recombinant segregants. The nutritional markers appear to be asymmetrically distributed around the map, the upper arc containing a larger proportion of genes. The arrangement of genes in the upper arc recalls that of S. coelicolor A3(2), with which S. rimosus forms fertile crosses. The ‘empty’ regions known in the S. coelicolor map may be absent in S. rimosus.
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