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Abstract
SUMMARY: Bacteroids isolated from nodules produced by one strain of Rhizobium japonicum (CC705) had strong nitrate-reducing activity and reduced C2H2 to C2H4 and N2 to NH3 anaerobically with nitrate. Bacteroids of another strain (CB1809) were much less active nitrate reducers and reduced little C2H2 anaerobically. Nitrite, which accumulated in the medium in anaerobic assays, was an inhibitor of C2H2 reduction in both aerobic and anaerobic conditions. Succinate, at about 25 mM, stimulated both nitrate reduction and C2H2 reduction under aerobic conditions. Glucose stimulated C2H2 reduction up to 120 mM but nitrate reduction was inhibited in the presence of glucose. In terms of electrons transferred, the aerobic pathway appeared to be about 2.5 times more efficient than the anaerobic pathway in supporting nitrogenase activity of CC705 bacteroids.
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