Summary: Two complementary frameshift mutations in the gene of have been isolated following recombination of the pseudowild double mutant with the wild-type strain. One of these mutations recombines with a third, distal frameshift mutation to give a slow-growing Trp strain. Consideration of the relevant RNA codon sequences suggests that the slow-growing recombinant should contain a unique tryptophan codon in the altered reading phase between the frameshift mutations. This prediction has been verified by analysis of the purified tryptophan synthetase A protein from the double mutant. The preferential synthesis of A protein normally associated with prolonged tryptophan starvation is eliminated in the strain which has a tryptophan residue in its A protein.


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