1887

Abstract

Summary: AMI, which can grow on C compounds, also grows well on pyruvate and lactate. Extracts of the pyruvate-grown organism catalysed the synthesis of only small amounts of phosphoeno/pyruvate directly from pyruvate. Experiments tracing the incorporation of radioactivity into bacterial constituents from pyruvate or lactate, each labelled in the C-1 or C-3 position, indicated that these substrates are mainly assimilated after removal of the C-1 atom. A mutant, C5, of AMI was isolated by a procedure intended to select organisms lacking phosphoeflo/pyruvate carboxylase. Although this enzyme was present in mutant C5, growth on lactate or pyruvate was impaired and there was no growth on C compounds or ethanol. Good growth on each of these substrates was restored by a supplement of glyoxylate or glycollate.

A scheme is proposed for the assimilation via malate synthase and phospho-pyruvate carboxykinase of the C fragment produced from pyruvate and lactate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-76-2-375
1973-06-01
2019-10-15
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-76-2-375
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error