Summary: The effects of the self-inhibitor, -dimethylguanosine, on the metabolism of spores of were investigated. It is concluded that since both self-inhibited and activated spores had similar initial values, the site of action of the self-inhibitor was not on respiratory metabolism. Incorporation of [UC]glucose revealed that organic acids and amino acids accumulated in self-inhibited spores and that protein synthesis was inhibited. Succinic acid accumulated only when the activated self-inhibited spores became anaerobic so its accumulation was not directly related to self-inhibition. Phenylalanine, methionine and tryptophan were absent in dormant spores but appeared after heat-activation and accumulated in self-inhibited spores. The accumulation of amino acids was related to the inability of self-inhibited spores to synthesize protein. analysis showed that protein synthesis began early in spore germination and that the self-inhibitor immediately arrested this process when added to activated spores and slowed it when added to germinating spores. No effect of self-inhibitor on RNA synthesis during germination was detected. Thus, we conclude that the self-inhibitor, a methylated base of guanine, regulated spore germination by preventing the synthesis of protein necessary for subsequent development.


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