RT Journal Article SR Electronic(1) A1 De Vries, O. M. H. A1 Wessels, J. G. H.YR 1973 T1 Release of Protoplasts from Schizophyllum commune by Combined Action of Purified α-1,3-Glucanase and Chitinase Derived from Trichoderma viride JF Microbiology, VO 76 IS 2 SP 319 OP 330 DO https://doi.org/10.1099/00221287-76-2-319 PB Microbiology Society, SN 1465-2080, AB Summary: Protoplast release from young mycelium of Schizophyllum commune by a lytic enzyme preparation from Trichoderma viride was accompanied by degradation of the three wall polymers, s-glucan, r-glucan and chitin. Part of the s-glucan was resistant. The resistance of s-glucan to enzymatic degradation increased with culture age and concomitantly the yield of protoplasts was reduced. Isolated s-glucan was also partly resistant to degradation, probably due to its crystallinity. In living cells s-glucan protected chitin and possibly r-glucan against degradation by external enzymes. s-glucanase, r-glucanase, chitinase and exo-laminarinase were purified from the Trichoderma enzyme mixture. Addition of only s-glucanase and chitinase was essential for protoplast release; r-glucan was degraded endogenously., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-76-2-319