Summary: Protoplast release from young mycelium of by a lytic enzyme preparation from was accompanied by degradation of the three wall polymers, -glucan, -glucan and chitin. Part of the -glucan was resistant. The resistance of -glucan to enzymatic degradation increased with culture age and concomitantly the yield of protoplasts was reduced. Isolated -glucan was also partly resistant to degradation, probably due to its crystallinity. In living cells -glucan protected chitin and possibly -glucan against degradation by external enzymes. -glucanase, -glucanase, chitinase and exo-laminarinase were purified from the Trichoderma enzyme mixture. Addition of only -glucanase and chitinase was essential for protoplast release; -glucan was degraded endogenously.


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