1887

Abstract

Summary: The conditions for induction of thymine deficiency by sulphonamides and their effect on viability were investigated in In minimal medium, sulphonamides led to a slight decrease in the viable count over a prolonged length of time. This decrease was more pronounced in Casamino acid supplemented medium. Concentrations of as low as 1 μg/ml of sulphamethoxazole or some other sulphonamides killed up to 99.9% of bacteria within 4 h after manifestation of inhibition in media supplemented by Casamino acids and inosine. No strict relation was found between sulphonamide concentration and the number of surviving bacteria. In all experiments sulphonamides exerted their effects after a time lag of 3 to 4 h. Addition of thymidine prevented death. Effects similar to those in media containing Casamino acids and inosine were demonstrated in growth medium containing human blood. The possible role of this kind of action of sulphonamides in chemotherapy is discussed.

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/content/journal/micro/10.1099/00221287-76-2-255
1973-06-01
2022-01-18
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References

  1. Adams J. M, Capecchi M. R. 1966; W-formylmethionyl-sRNA as the initiator of protein synthesis. Proceedings of the National Academy of Sciences of the United States of America 55:147–155
    [Google Scholar]
  2. Angehrn P., Then R. 1973; Nature of trimethoprim-induced death in Escherichia coli. Arzneimittel-Forschung (Drug Research) (in the press)
    [Google Scholar]
  3. Bünger P., Diller W., Führ J., Krüger-Thiemer E. 1961; Vergleichende Untersuchungen an neueren Sulfonamiden. Arzneimittel-Forschung (Drug Research) 11:247–255
    [Google Scholar]
  4. Bushby S. R. M., Hitchings G. H. 1968; Trimethoprim, a sulphonamide potentiator. British Journal of Pharmacology and Chemotherapy 33:72–90
    [Google Scholar]
  5. Capecchi M. R. 1966; Initiation of E. coli proteins. Proceedings of the National Academy of Sciences of the United States of America 55:1517–1524
    [Google Scholar]
  6. Clark B. F. C., Marcker K. A. 1966; The role of N-formyl-methionyl-sRNA in protein biosynthesis. Journal of Molecular Biology 17:394–406
    [Google Scholar]
  7. Cohen S. S., Barner H. D. 1956; Studies on the induction of thymine deficiency and on the effects of thymine and thymidine analogues in Escherichia coli . Journal of Bacteriology 71:588–597
    [Google Scholar]
  8. Cummings D. J., Kusy A. R. 1969; Thymineless death in Escherichia coli: inactivation and recovery. Journal of Bacteriology 99:558–566
    [Google Scholar]
  9. Cummings D. J., Kusy A. R. 1970; Thymineless death in Escherichia coli: deoxyribonucleic acid replication and the immune state. Journal of Bacteriology 102:106–117
    [Google Scholar]
  10. Cummings D. J., Mondale L. 1967; Thymineless death in Escherichia coli: strain specificity. Journal of Bacteriology 93:1917–1924
    [Google Scholar]
  11. Deutch C. E., Pauling C. 1971; Survival and macromolecular synthesis during incubation of Escherichia coli in limiting thymine. Journal of Bacteriology 106:197–203
    [Google Scholar]
  12. Ganguli N., Bhattacharjee S. B. 1968; Influence of chloramphenicol on thymineless death in bacteria. Biochimica et biophysica acta 169:545–547
    [Google Scholar]
  13. Garrett E. R., Wright O. K. 1967; Kinetics and mechanisms of action of drugs on microorganisms. VI. Quantitative adherence of sulphonamide action on microbial growth to a receptor-site model. Journal of Pharmaceutical Sciences 56:1576–1585
    [Google Scholar]
  14. Neipp L., Sackmann W., Tripod J. 1961; Some new trends in the field of experimental research on sulphonamides. Antibiotica et Chemotherapia 9: pp 19–82
    [Google Scholar]
  15. Seydel J. K. 1966; Molekulare Grundlagen der Sulfonamidwirkung. Arzneimittel-Forschung (Drug Research) 16:1447–1453
    [Google Scholar]
  16. Seydel J. K. 1968; Sulfonamides, structure-activity relationship and mode of action. Journal of Pharmaceutical Sciences 57:1455–1478
    [Google Scholar]
  17. Streeter A. M., O’Neill B. J., Shum H. Y. 1970; Folic acid deficiency in Lactobacillus casei . Pathology 2:217–221
    [Google Scholar]
  18. Then R., Angehrn P. 1973; Effects of trimethoprim on Escherichia coli under limited nutrition. Arzneimittel-Forschung (Drug Research) (in the press)
    [Google Scholar]
  19. Woods D. D. 1962; The biochemical mode of action of the sulphonamide drug. Journal of General Microbiology 29:687–702
    [Google Scholar]
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