1887

Abstract

Summary: Treatment of growing with hydroxyurea decreased the DNA/protein ratio and markedly increased the specific activities of DNA polymerase and ATP-dependent deoxyribonuclease. These changes were similar to those produced by iron limitation of growth. Treating with nalidixic acid also decreased the DNA/protein ratio but had no effect on the activity of either enzyme. Growth of under conditions of zinc limitation did not diminish the specific activity of DNA polymerase thus not favouring a role for zinc in the action of this enzyme. The activity of the ATP-dependent deoxyribonuclease was, however, slightly increased. In previous reports the specific activity of DNA polymerase in this organism has been seriously underestimated due to interfering deoxyribonuclease and nucleoside triphosphatase. This was corrected by sufficient dilution of the extract. Revised specific activities of DNA polymerase in carbon-limited, nitrogen-limited and iron-limited cultures are, respectively, 10, 14, 11 and 64 units/mg protein.

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/content/journal/micro/10.1099/00221287-76-1-189
1973-05-01
2022-01-19
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References

  1. Cooper P. K., Hanawalt P. C. 1972; Role of DNA polymerase I and the rec system in excision-repair in Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 69:1156–1160
    [Google Scholar]
  2. Crerar M., Pearlman R. E. 1971; DNA polymerase activity from Tetrahymena pyriformis . FEBS Letters 18:231–237
    [Google Scholar]
  3. Freifelder D. 1969; Single-strand breaks in bacterial DNA associated with thymine starvation. Journal of Molecular Biology 45:1–7
    [Google Scholar]
  4. Gale G. R., Kendall S. M., McLain H. H., DuBois S. 1964; Effect of hydroxyurea on Pseudomonas aeruginosa . Cancer Research 24:1012–1016
    [Google Scholar]
  5. Gold M., Hurwitz J. 1963; The enzymatic methylation of the nucleic acids. Cold Spring Harbor Symposia on Quantitative Biology 28:149–156
    [Google Scholar]
  6. Goss W. A., Deitz W. H., Cook T. M. 1964; Mechanism of action of nalidixic acid on Escherichia coli . Journal of Bacteriology 88:1112–1118
    [Google Scholar]
  7. Goss W. A., Deitz W. A., Cook T. M. 1965; Mechanism of action of nalidixic acid on Escherichia coli . Journal of Bacteriology 89:1068–1074
    [Google Scholar]
  8. Holliday R. 1971; Biochemical measure of the time and frequency of radiation-induced allelic recombination in Ustilago . Nature New Biology 232:233–236
    [Google Scholar]
  9. Huberman J. A., Kornberg A. 1970; Enzymatic synthesis of deoxyribonucleic acid. XXXV. A - 3’-hydroxylribonucleotide binding site of Escherichia coli deoxyribonucleic acid polymerase. Journal of Biological Chemistry 245:5326–5334
    [Google Scholar]
  10. Keiding J., Westergaard O. 1971; Induction of DNA polymerase activity in irradiated Tetrahymena cells. Experimental Cell Research 64:317–322
    [Google Scholar]
  11. Kelly R. B., Atkinson M. R., Huberman J. A., Kornberg A. 1969; Excision of thymine dimers and other mismatched sequences by DNA polymerase of Escherichia coli . Nature, London 224:495–501
    [Google Scholar]
  12. Krakoff I. H., Brown N. C., Reichard P. 1968; Inhibition of ribonucleoside diphosphate reductase by hydroxyurea. Cancer Research 28:1559–1565
    [Google Scholar]
  13. Litman R. M. 1968; A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose. Journal of Biological Chemistry 243:6222–6233
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  15. Luzzati D. 1966; Effect of thymine starvation on messenger ribonucleic acid synthesis in Escherichia coli . Journal of Bacteriology 92:1435–1446
    [Google Scholar]
  16. McNulty M. S., Winder F. G. 1971; Partial purification and properties of a DNA polymerase from Mycobacterium smegmatis . Biochimica et biophysica acta 254:213–225
    [Google Scholar]
  17. Moore E. C. 1969; The effects of ferrous ion and dithioerythritol on inhibition by hydroxyurea of ribonucleotide reductase. Cancer Research 29:291–295
    [Google Scholar]
  18. Neuhard J. 1967; Studies on the acid-soluble nucleotide pool in Escherichia coli. IV. Effects of hydroxyurea. Biochimica et biophysica acta 145:1–6
    [Google Scholar]
  19. Okazaki T., Kornberg A. 1964; Enzymatic synthesis of deoxyribonucleic acid. XV. Purification and properties of a polymerase from Bacillus subtilis . Journal of Biological Chemistry 239:259–268
    [Google Scholar]
  20. Ratledge C., Winder F. G. 1964; Effect of iron and zinc on growth patterns of Escherichia coli in an iron-deficient medium. Journal of Bacteriology 87:823–827
    [Google Scholar]
  21. Richardson C. C., Schildkraut C. L., Aposhian H. V., Kornberg A. 1964; Enzymatic synthesis of deoxyribonucleic acid. XIV. Further purification and properties of deoxyribonucleic acid polymerase of Escherichia coli . Journal of Biological Chemistry 239:222–232
    [Google Scholar]
  22. Rosenkranz H. S., Levy J. A. 1965; Hydroxyurea: a specific inhibitor of deoxyribonucleic acid synthesis. Biochimica et biophysica acta 95:181–183
    [Google Scholar]
  23. Rupp D. W., Wilde C. E. III, Reno D. L., Howard-Flanders P. 1971; Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli . Journal of Molecular Biology 61:25–44
    [Google Scholar]
  24. Slater J. P., Mildvan A. S., Loeb L. A. 1971; Zinc in DNA polymerases. Biochemical and Biophysical Research Communications 44:37–43
    [Google Scholar]
  25. Walker J. R. 1970; Thymine starvation and single-strand breaks in chromosomal deoxyribonucleic acid in Escherichia coli . Journal of Bacteriology 104:1391–1392
    [Google Scholar]
  26. Winder F. G., Coughlan M. P. 1969; A nucleoside triphosphate-dependent deoxyribonucleic acid-breakdown system in Mycobacterium smegmatis, and the effect of iron limitation on the activity of this system. Biochemical Journal 111:679–687
    [Google Scholar]
  27. Winder F. G., Coughlan M. P. 1971; Comparison of the effects of carbon, nitrogen and iron limitation on the growth and on the RNA and DNA content of Mycobacterium smegmatis . Irish Journal of Medical Science 140:16–25
    [Google Scholar]
  28. Winder F. G., Lavin M. F. 1971; Partial purification and properties of a nucleoside triphosphate-dependent deoxyribonuclease from Mycobacterium smegmatis . Biochimica et biophysica acta 247:542–561
    [Google Scholar]
  29. Winder F. G., McNulty M. S. 1970; Increased DNA polymerase activity accompanying decreased DNA content in iron-deficient Mycobacterium smegmatis . Biochimica et biophysica acta 209:578–586
    [Google Scholar]
  30. Winder F. G., O’Hara C. 1962; Effects of iron deficiency and of zinc deficiency on the composition of Mycobacterium smegmatis . Biochemical Journal 82:98–108
    [Google Scholar]
  31. Winder F. G., O’Hara C. 1964; Effects of iron deficiency and of zinc deficiency on the activities of some enzymes in Mycobacterium smegmatis . Biochemical Journal 90:122–126
    [Google Scholar]
  32. Zimmerman B. K. 1966; Purification and properties of deoxyribonucleic acid polymerase of Micrococcus lysodeikticus . Journal of Biological Chemistry 241:2035–2041
    [Google Scholar]
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