1887

Abstract

Summary: The isolation of a group of phages attacking the actinomycete A3(2) is described. One of the phages, VP 11, has been characterized, with emphasis on the ways in which it might aid studies on the host organism. VP 11 is a virulent DNA phage with a wide host range. It has a latent period of 40 min at 30°C and an average burst size of about 40. The phage has been examined electron-micro-scopically and found to belong in group B of Bradley’s morphological classification. It has also been shown to be host-cell reactivated after u.v.-irradiation. Two temperature-sensitive mutations have been shown to undergo recombination. The phage requires Ca for stability and is very sensitive to chelating agents. Some of the difficulties encountered in using a mycelial organism as a host in phage studies are described and discussed.

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1973-05-01
2024-04-24
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References

  1. Adams M. H. 1959 Bacteriophages New York: Wiley-Interscience;
    [Google Scholar]
  2. Bassel A., Shaw M., Campbell L. L. 1971; Dissociation by chelating agents and substructure of the thermophilic bacteriophage TP 84. Journal of Virology 7:663–672
    [Google Scholar]
  3. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31:230–314
    [Google Scholar]
  4. Bradley S. G., Anderson D. L. 1958; Taxonomic implication of actinophage host-range. Science, New York 128:413–414
    [Google Scholar]
  5. Bradley S. G., Anderson D. L., Jones L. A. 1961; Phylogeny of Actinomycetes as revealed by susceptibility to actinophage. Developments in Industrial Microbiology 2:223–237
    [Google Scholar]
  6. Carvajal F. 1955; Host-parasite relations with a polyvalent streptomycophage from Streptomyces griseus . Antibiotics and Chemotherapy 5:28–32
    [Google Scholar]
  7. Chater K. F. 1972; A morphological and genetic mapping study of white colony mutants of Streptomyces coelicolor . Journal of General Microbiology 72:9–28
    [Google Scholar]
  8. Chater K. F., Hopwood D. A. 1973; Differentiation in Actinomycetes. Symposium of the Society for General Microbiology 23:143–160
    [Google Scholar]
  9. Collard C. A. 1970; Etude comparative des méthodes d’isolement d’actinophages à partir de leurs habitats naturels. Comptes rendues des séances de la Société de Biologie 164:465–468
    [Google Scholar]
  10. Dowding J. E. 1972 Studies on bacteriophages of Streptomyces coelicolor Ph. D. Thesis, University of East Anglia; Norwich:
    [Google Scholar]
  11. Edgar R. S. 1963; Some technical considerations concerning experiments on phage recombination. In Methodology in Basic Genetics pp 19–36 Edited by Burdette W. J. San Francisco: Holden Day;
    [Google Scholar]
  12. Edgar R. S., Denhardt G. H., Epstein R. H. 1964; A comparative genetic study of conditional lethal mutations of bacteriophage T4D. Genetics 49:635–648
    [Google Scholar]
  13. Glover S. W. 1962; Valine-resistant mutants of Escherichia coli k-12. Genetical Research 3:448–460
    [Google Scholar]
  14. Harold R. J., Hopwood D. A. 1970a; Ultraviolet-sensitive mutants of Streptomyces coelicolor. I. Phenotypic characterisation. Mutation Research 10:427–438
    [Google Scholar]
  15. Harold R. J., Hopwood D. A. 1970b; Ultraviolet-sensitive mutants of Streptomyces coelicolor. II. Genetics. Mutation Research 10:439–448
    [Google Scholar]
  16. Hopwood D. A. 1967; Genetic analysis and genome structure in Streptomyces coelicolor . Bacteriological Reviews 31:373–403
    [Google Scholar]
  17. Hopwood D. A., Sermonti G. 1962; The genetics of Streptomyces coelicolor. Advances in Genetics 11:273–342
    [Google Scholar]
  18. Hopwood D. A., Wildermuth H., Palmer H. M. 1970; Mutants of Streptomyces coelicolor defective in sporulation. Journal of General Microbiology 61:397–408
    [Google Scholar]
  19. Hütter R. 1967 Systematik der Streptomyceten Basel: Karger-Verlag;
    [Google Scholar]
  20. Kutzner H. J., Waksman S. A. 1959; Streptomyces coelicolor Muller and Streptomyces violaceoruber Waksman and Curtis, two distinctly different organisms. Journal of Bacteriology 78:528–538
    [Google Scholar]
  21. Lomovskaya N. D., Emeljanova L. K., Alikhanian S. I. 1971; The genetic location of prophage on the chromosome of Streptomyces coelicolor . Genetics 68:341–347
    [Google Scholar]
  22. Lomovskaya N. D., Mkrtumian N. M., Gostimskaya N. L., Danilenko V. N. 1972; Characterization of temperate actinophage øC 31 isolated from Streptomyces coelicolor a3(2). Journal of Virology 9:258–262
    [Google Scholar]
  23. Pridham T. G., Hesseltine C. W., Benedict R. G. 1958; A guide for the classification of streptomycetes according to selected groups. Placement of strains in morphological sections. Applied Microbiology 6:52–79
    [Google Scholar]
  24. Ritzi D., Bradley S. G., Jones L. A. 1968; Ultrastructure of phage-infected Streptomyces venezuelae . Developments in Industrial Microbiology 9:235–241
    [Google Scholar]
  25. Studier F. W. 1969; The genetics and physiology of bacteriophage T 7. Virology 39:562–574
    [Google Scholar]
  26. Tresner H. D., Backus E. J. 1963; System of color wheels for streptomycete taxonomy. Applied Microbiology 11:335–338
    [Google Scholar]
  27. Van Alstyne M. H., Otto R. H., McCoy E. 1955; Characteristics of Streptomyces griseus strains resistant to phage. Journal of Bacteriology 70:113–119
    [Google Scholar]
  28. Vivian A. 1971; Genetic control of fertility in Streptomyces coelicolor A3(2): plasmid involvement in the interconversion of uf and if strains. Journal of General Microbiology 69:353–364
    [Google Scholar]
  29. Welsch M. 1956; Influence de Page d’un Streptomyces sur ses réactions à un actinophage. Comptes rendues des seances de la Société de Biologie 150:609–613
    [Google Scholar]
  30. Welsch M. 1969; Biology of actinophages. Symposium on Genetics and Breeding of Streptomyces, Dubrovnik pp 43–62 Zagreb: Yugoslav Academy of Sciences and Arts;
    [Google Scholar]
  31. Welsch M., Minon A., Schönfeld J. K. 1955; Isolation of actinophages. Experientia 11:24–25
    [Google Scholar]
  32. Welsch M., Rutten-Pinckaers A., Selman M. 1963; Recherches sur des Streptomyces d’Afrique Centrale. IV. Isoantibiose, lysogénie et actinophages. Bulletin de la Société royale des sciences de Liégé 32:529–573
    [Google Scholar]
  33. Wieringa K. T., Wiebols G. L. W. 1936; De aardappelschurft en de heterolyse des schurftparasiet. Tijdschrift over Plantenziekten 42:235–240
    [Google Scholar]
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