1887

Abstract

Summary: Conditions have been investigated which allow the mapping of the replication order of the genes on the pneumococcal chromosome by the density-shift method. Adequate synchrony of DNA replication was achieved by using cultures already involved in the strict regime designed to give competent cells; such cultures were grown for 30 min into the stationary phase and then diluted into the medium in which the density-shift will occur. The shift was achieved by changing from either (i) ‘light’ peptone medium to synthetic medium containing 5-BU, or (ii) peptone medium prepared in DO to ‘light’ peptone medium. Samples of such cultures were lysed and centrifuged in caesium chloride and the gradients so formed fractionated and analysed for the transforming activity for the genetic markers. A map giving the order of replication of eleven markers is described.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-76-1-101
1973-05-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/76/1/mic-76-1-101.html?itemId=/content/journal/micro/10.1099/00221287-76-1-101&mimeType=html&fmt=ahah

References

  1. Avery O. T., MacLeod C. M., McCarty M. 1944; Studies on the chemical nature of the substance inducing transformation of pneumococcal types. Induction of transformation by a desoxyribonucleic acid fraction isolated from Pneumococcus type III. Journal of Experimental Biology 89:137–157
    [Google Scholar]
  2. Butler L. O. 1965; A co-precipitation method for the preparation of transforming DNA from small samples of low density bacterial cultures. Journal of General Microbiology 39:247–252
    [Google Scholar]
  3. Butler L. O. 1970; Sintesi dell’ocido desossiribonucleico nei batteri. Recenti Progressi in Medicina 49:303–326
    [Google Scholar]
  4. Butler L. O., Smiley M. B. 1970; Characterization by transformation of an ampicillin-resistant mutant of pneumococcus. Journal of General Microbiology 61:189–195
    [Google Scholar]
  5. Cairns J. 1963a; The bacterial chromosome and its manner of replication as seen by autoradiography. Journal of Molecular Biology 6:208–213
    [Google Scholar]
  6. Cairns J. 1963b; The chromosome of Escherichia coli . Cold Spring Harbor Symposia of Quantitative Biology 28:43–46
    [Google Scholar]
  7. Caro L. G., Berg C. M. 1968; Chromosome replication in some strains of Escherichia coli k12. Cold Spring Harbor Symposium of Quantitative Biology 33:559–573
    [Google Scholar]
  8. Caro L. G., Berg C. M. 1969; Chromosome replication in Escherichia coli II. Origin of replication in F and F+ strains. Journal of Molecular Biology 45:325–336
    [Google Scholar]
  9. Cutler R. G., Evans J. E. 1966; Synchronisation of bacteria by a stationary-phase method. Journal of Bacteriology 91:469–476
    [Google Scholar]
  10. Dubnau D., Goldthwaite C., Smith I., Marmur J. 1967; Genetic mapping in Bacillus subtilis . Journal of Molecular Biology 27:163–185
    [Google Scholar]
  11. Dubnau D., Smith I., Marmur J. 1965; Gene conservation in Bacillus species. II. The location of genes concerned with the synthesis of ribosomal components and soluble RNA. Proceedings of the National Academy of Sciences of the United States of America 54:724–730
    [Google Scholar]
  12. Ephrussi-Taylor H. 1951; Transformations allogenes du pneumocoque. Experimental Cell Research 2:589–607
    [Google Scholar]
  13. Ephrussi-Taylor H. 1960; L’état du DNA transformant au cours des premieres phases de la transformation bacterienne. Comptes rendus des seances de la Société de biologie 154:1951–1955
    [Google Scholar]
  14. Ephrussi-Taylor H. 1961; Étude de la recombinason à l’échelle moléculaire de la transformation bactérienne. Journal de chimie physique et de physico-chemie biologique 58:1090–1099
    [Google Scholar]
  15. Firshein W., Schwenzfeier W. C. 1969; Characterization of excess deoxyribonucleic acid synthesized by pneumococci in the presence of polyadenylic acid and deoxyribonucleic acid precursors. Journal of Bacteriology 97:1106–1113
    [Google Scholar]
  16. Louarn J-M., Sicard A. M. 1969; Identical transformability of both strands of recipient DNA in Diplococcus pneumoniae . Biochemical and Biophysical Research Communications 36:101–109
    [Google Scholar]
  17. Marmur J., Schildkraut C. L. 1961; Growth of bacteria labelled with heavy isotopes for the isolation of nucleic acids. Nature, London 189:636–638
    [Google Scholar]
  18. Martin C., Ephrussi-Taylor H. 1964; Effects of partial deuteration on transforming DNA of pneumococcus. Biochemica and biophysica acta 80:411–421
    [Google Scholar]
  19. Masters M., Broda P. 1971; Evidence for the bidirectional replication of the Escherichia coli chromosome. Nature New Biology 232:137–140
    [Google Scholar]
  20. Nagata T. 1963; The molecular synchrony and sequential replication of DNA in Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 49:551–559
    [Google Scholar]
  21. Nishioka Y., Eisenstark A. 1970; Sequence of genes replicated in Salmonella typhimurium as examined by transduction techniques. Journal of Bacteriology 102:320–333
    [Google Scholar]
  22. Oishi M., Oishi A., Sueoka N. 1966; Location of genetic loci of soluble RNA on Bacillus subtilis chromosome. Proceedings of the National Academy of Sciences of the United States of America 55:1095–1103
    [Google Scholar]
  23. Oishi M., Sueoka N. 1965; Location of genetic loci of ribosomal RNA on Bacillus subtilis chromosome. Proceedings of the National Academy of Sciences of the United States of America 54:483–491
    [Google Scholar]
  24. Oishi M., Yoshikawa H., Sueoka N. 1964; Synchronous and dichotomous replications of the Bacillus subtilis chromosome during spore germination. Nature, London 204:1069–1073
    [Google Scholar]
  25. O’Sullivan A., Sueoka N. 1967; Sequential replication of Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiments. Journal of Molecular Biology 27:349–368
    [Google Scholar]
  26. Sicard A. M. 1964; A new synthetic medium for Diplococcus pneumoniae and its use for the study of reciprocal transformation at the ami A locus. Genetics 50:31–44
    [Google Scholar]
  27. Sicard A. M., Ephrussi-Taylor H. 1965; Genetic recombination in DNA-induced transformation of pneumococcus. II. Mapping the amiA region. Genetics 52:1207–1227
    [Google Scholar]
  28. Wake R. G. 1972; Visualisation of re-initiated chromosomes in Bacillus subtilis . Journal of Molecular Biology 68:501–509
    [Google Scholar]
  29. Yahara I. 1971; On the replication of Escherichia coli chromosome. Journal of Molecular Biology 57:373–376
    [Google Scholar]
  30. Yoshikawa H., Sueoka N. 1963a; Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phase. Proceedings of the National Academy of Sciences of the United States of America 49:559–566
    [Google Scholar]
  31. Yoshikawa H., Sueoka N. 1963b; Sequential replication in Bacillus subtilis chromosome. II. Isotopic transfer experiments. Proceedings of the National Academy of Sciences of the United States of America 49:806–813
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-76-1-101
Loading
/content/journal/micro/10.1099/00221287-76-1-101
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error