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SUMMARY: RNA was isolated from vegetative Myxococcus xanthus FB and myxospores. Organisms were labelled with [32P]orthophosphate during encystment and the metabolic stability of the radioactive RNA was followed by fractionating purified RNA from ‘pulse-chased’ cultures by polyacrylamide gel electrophoresis. Much of the label was in heterodisperse (messenger-like) RNA but the stable RNA (present in the mature myxospores) was largely high-molecular weight ribosomal RNA and its precursors.
The role of RNA synthesis in the morphogenetic cycle of Myxococcus xanthus is discussed in the light of these data and of the properties of the ribosomes and ribosomal proteins from this organism.
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