1887

Abstract

SUMMARY: RNA was isolated from vegetative FB and myxospores. Organisms were labelled with [P]orthophosphate during encystment and the metabolic stability of the radioactive RNA was followed by fractionating purified RNA from ‘pulse-chased’ cultures by polyacrylamide gel electrophoresis. Much of the label was in heterodisperse (messenger-like) RNA but the stable RNA (present in the mature myxospores) was largely high-molecular weight ribosomal RNA and its precursors.

The role of RNA synthesis in the morphogenetic cycle of is discussed in the light of these data and of the properties of the ribosomes and ribosomal proteins from this organism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-75-2-401
1973-04-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/75/2/mic-75-2-401.html?itemId=/content/journal/micro/10.1099/00221287-75-2-401&mimeType=html&fmt=ahah

References

  1. Bacon K., Rosenberg E. 1967; Ribonucleic acid synthesis during morphogenesis in Myxococcus xanthus. Journal of Bacteriology 94:1883–1889
    [Google Scholar]
  2. Foster H. A., Parish J. H. 1973; Ribosomes, ribosomal subunits and ribosomal proteins from Myxococcus xanthus. Journal of General Microbiology 75:391–400
    [Google Scholar]
  3. Hecht N. B., Woese C. R. 1968; Separation of bacterial ribosomal ribonucleic acid from its macromolecular precursors by polyacrylamide gel electrophoresis. Journal of Bacteriology 95:986–990
    [Google Scholar]
  4. Kirby K. S., Fox-Carter E., Guest M. 1967; Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria. Biochemical Journal 104:258–262
    [Google Scholar]
  5. Loening U. E. 1968; The fractionation of high molecular weight RNA. In Chromatographic and Electrophoretic Techniques. Zone Electrophoresis vol 2 pp 437–442 Edited by Smith I. London: Heinemann;
    [Google Scholar]
  6. Losick R., Sonenshein A. L. 1969; Change in the template specificity of RNA polymerase during sporulation in Bacillus subtilis. Nature, London 224:35–38
    [Google Scholar]
  7. Okano P., Bacon K., Rosenberg E. 1972; Ribonucleic acid synthesis during microcyst formation in Myxococcus xanthus: Characterization by deoxyribonucleic acid-ribonucleic acid hybridization. Journal of Bacteriology 104:275–282
    [Google Scholar]
  8. Parish J. H. 1968; Use of DEAE-cellulose for the chromatography of RNA of high molecular weight. Biochimica et biophysica acta 169:14–20
    [Google Scholar]
  9. Parish J. H. 1972; Isolation and fractionation of RNA. In Subcellular Components 2nd ed. pp 251–278 Edited by Birnie G. D. London: Butterworths;
    [Google Scholar]
  10. Parish J. H., Khairul Bashar S. A. M., Brown N. L., Brown M. 1971; Cell-free synthesis of tryptophanase from Escherichia coli. Use of ribonucleic acid isolated from induced cells and a comparison of the product from a system employing ribosomes and exogenous ribonucleic acid. Biochemical Journal 125:643–653
    [Google Scholar]
  11. Parish J. H., Kirby K. S. 1966; Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochemica et biophysica acta 129:554–562
    [Google Scholar]
  12. Ramsay W. S., Dworkin M. 1968; Microcyst germination in Myxococcus xanthus. Journal of Bacteriology 95:2249–2257
    [Google Scholar]
  13. Ramsay W. S., Dworkin M. 1970; Stable messenger ribonucleic acid and germination of Myxococcus xanthus microcysts. Journal of Bacteriology 101:531–540
    [Google Scholar]
  14. Rosenberg E., Katarski M., Gottlieb P. 1967; Deoxyribonucleic acid synthesis during exponential growth and microcyst formation in Myxococcus xanthus. Journal of Bacteriology 93:1402–1408
    [Google Scholar]
  15. Sadler W., Dworkin M. 1966; Induction of cellular morphogenesis in Myxococcus xanthus. II. Macromolecular synthesis and mechanism of inducer action. Journal of Bacteriology 91:1520–1525
    [Google Scholar]
  16. Sakakibara Y., Tanooka H., Terano H. 1970; Defined conditions for DNA extraction from Bacillus subtilis spores. Biochimica et biophysica acta 199:549–550
    [Google Scholar]
  17. Uchida T., Egami F. 1967; Ribonuclease T1 from takadiastase. In Methods in Enzymology vol xii a pp 228–239 Edited by Grossman L., Moldave K. New York and London: Academic Press;
    [Google Scholar]
  18. Zusman D., Gottlieb P., Rosenberg E. 1971; Division cycle of Myxococcus xanthus. III. Kinetics of cell growth and protein synthesis. Journal of Bacteriology 105:811–819
    [Google Scholar]
  19. Zusman D., Rosenberg E. 1970; DNA cycle and Myxococcus xanthus. Journal of Molecular Biology 49:609–619
    [Google Scholar]
  20. Zusman D., Rosenberg E. 1971; Division cycle of Myxococcus xanthus. II. Kinetics of stable and unstable ribonucleic acid synthesis. Journal of Bacteriology 105:801–810
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-75-2-401
Loading
/content/journal/micro/10.1099/00221287-75-2-401
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error