A Respiratory-deficient Mutant in the Obligately Aerobic Yeast Free

Abstract

SUMMARY: A glycerol-negative respiratory-deficient mutant is described which is not as judged by several criteria: (i) it grows slowly on glycerol; (ii) it reverts to glycerol-positive; (iii) it grows slowly on other non-fermentable carbon sources; and (iv) it has a normal cytochrome spectrum. It is not an oxidative phosphorylation mutant because the growth yield on glucose is the same as the parent. This mutant is specifically blocked in glycerol respiration but, in addition, is pleiotropic, affecting growth rates on other carbon sources, sporulation and diploid mitotic segregational frequencies of other genes.

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/content/journal/micro/10.1099/00221287-75-2-377
1973-04-01
2024-03-28
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References

  1. Claisse M. L., Pere-Aubert G. A., Clavilier L. P., Slonimsky P. P. 1970; Methode d’estimation de la concentration des cytochromes dans les cellules entieres de levure. European Journal of Biochemistry 16:430–438
    [Google Scholar]
  2. Crandall M. 1973; Comparison of Hansenula wingei, a petite-negative, obligately aerobic yeast, to the petite-positive yeast Saccharomyces cerevisiae. Journal of General Microbiology 75:365–375
    [Google Scholar]
  3. Gancedo C., Gancedo J. M., Sols A. 1968; Glycerol metabolism in yeasts. European Journal of Biochemistry 5:165–172
    [Google Scholar]
  4. Mori S., Nakai S. 1968; Induction of allelic recombination in UV and X-ray sensitive mutants of yeast. Proceedings of the XII International Congress of Genetics 1:80
    [Google Scholar]
  5. Resnick M. A. 1969; Genetic control of radiation sensitivity in Saccharomyces cerevisiae. Genetics 62:519–531
    [Google Scholar]
  6. Slonimski P. P. 1949; Action de l’acriflavine sur les levures. IV. Model d’utilisation du glucose par les mutants ‘petite colonies’. Annals de l’Institut Pasteur 76:510–530
    [Google Scholar]
  7. Slonimski P. P. 1953 Formation des enzymes respiratoires chez le levure Paris: Masson;
    [Google Scholar]
  8. Somlo M. 1968; Induction and repression of mitochondrial ATPase in yeast. European Journal of Biochemistry 5:276–284
    [Google Scholar]
  9. Somlo M. 1971; Oxidative phosphorylation in intact cells of normal and op1 mutant yeast undergoing respiratory adaptation. Biochimie 53:819–830
    [Google Scholar]
  10. Stickland L. H. 1951; The determination of small quantities of bacteria by means of the biuret reaction. Journal of General Microbiology 5:698–703
    [Google Scholar]
  11. Wickerham L. J. 1970; Hansenula H. et P. Sydow. In The Yeasts: A Taxonomic Study pp 226–315 Edited by Lodder J. Amsterdam: North Holland Publishing Co;
    [Google Scholar]
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