SUMMARY: A glycerol-negative respiratory-deficient mutant is described which is not petite as judged by several criteria: (i) it grows slowly on glycerol; (ii) it reverts to glycerol-positive; (iii) it grows slowly on other non-fermentable carbon sources; and (iv) it has a normal cytochrome spectrum. It is not an oxidative phosphorylation mutant because the growth yield on glucose is the same as the parent. This glp mutant is specifically blocked in glycerol respiration but, in addition, is pleiotropic, affecting growth rates on other carbon sources, sporulation and diploid mitotic segregational frequencies of other genes.
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