1887

Abstract

SUMMARY: After extensive ethidium bromide (EB) treatment or ultraviolet irradiation or mutants could be isolated from the obligate aerobe -negativity is correlated with a lack of glucose repression. issensitive to EB on fermentable as well as non-fermentable carbon sources. Growth arrest by EB of single cells on agar is immediate but one or two divisions occur in liquid before the sensitive cells stop growing. Although EB treatment of the sensitive wild-type does not result in either mutagenesis or cell killing, there is a reversible inhibition of growth observed when EB-treated cells are plated on non-crmentable substrates. Mutants resistant to EB were isolated on either glucose + EB or glycerol + EB plates. Mutants resistant to EB in glycerol medium are also (csistant to EB in glucose medium. However, mutants isolated on glucose + EB medium are still sensitive to EB in glycerol medium. When mutants resistant to EB in glycerol medium revert to sensitivity they retain resistance to EB in glucose medium. Resistance to EB in glucose medium is recessive in the diploid.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-75-2-363
1973-04-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/75/2/mic-75-2-363.html?itemId=/content/journal/micro/10.1099/00221287-75-2-363&mimeType=html&fmt=ahah

References

  1. Bolotin M., Coen D., Deutsch J., Dujon B., Netter P., Petrochilo E., Slonimski P. P. 1971; La récombinaison des mitochondries chez Saccharomyces cerevisiae. Bulletin de l’lnstitut Pasteur 69:215–239
    [Google Scholar]
  2. Brock T. D. 1961; Physiology of the conjugation process in the yeast Hansenula wingei. Journal of General Microbiology 26:487–497
    [Google Scholar]
  3. Bulder C. J. E. A. 1964a; Induction of petite mutation and inhibition of synthesis of respiratory enzymes in various yeasts. Antonie van Leeuwenhoek. Journal of Microbiology and Serology 30:1–9
    [Google Scholar]
  4. Bulder C. J. E. A. 1964b; Lethality of the petite mutation in petite negative yeasts. Antonie van Leeuwenhoek. Journal of Microbiology and Serology 30:442–454
    [Google Scholar]
  5. Coen D., Deutsch J., Netter P., Petrochilo E., Slonimski P. P. 1970; Mitochondrial genetics. I. Methodology and Phenomenology. In Control of Organelle Development pp 449–496 Symposium of the Society for Experimental Biology London: Cambridge University Press;
    [Google Scholar]
  6. Conti S. F., Brock T. D. 1965; Electron microscopy of the cell fusion in conjugating Hansenula wingei. Journal of Bacteriology 90:524–533
    [Google Scholar]
  7. Crandall M. 1973; A respiratory-deficient mutant in the obligately aerobic yeast Hansenula wingei. Journal of General Microbiology 75:377–381
    [Google Scholar]
  8. Crandall M., Brock T. D. 1968; Molecular basis of mating in the yeast Hansenula wingei. Bacteriological Reviews 32:139–163
    [Google Scholar]
  9. De Deken R. H. 1966a; The Crabtree effect: a regulatory system in yeast. Journal of General Microbiology 44:149–156
    [Google Scholar]
  10. De Deken R. H. 1966b; The Crabtree effect and its relation to the petite mutation. Journal of General Microbiology 44:157–165
    [Google Scholar]
  11. Ephrussi B., Hottinguer H. 1951; Cytoplasmic constituents of heredity. On an unstable cell state in yeast. Cold Spring Harbor Symposium of Quantitative Biology 16:75–85
    [Google Scholar]
  12. Goldring E. S., Grossman L. I., Marmur J. 1971; Petite mutation in yeast. II. Isolation of mutants containing mitochondrial deoxyribonucleic acid of reduced size. Journal of Bacteriology 107:377–381
    [Google Scholar]
  13. Kellerman G. M., Biggs D. R., Linnane A. W. 1969; Biogenesis of mitochondria. XI. A comparison of the effect of growth-limiting oxygen tension, intercalating agents, and antibiotics on the obligate aerobe Candida parapsilosis. Journal of Cell Biology 42:378–391
    [Google Scholar]
  14. Luha A., Sacroe L. E., Whittaker P. A. 1971; Biosynthesis of yeast mitochondria. Drug effects on the petive negative yeast Kluyveromyces lactis. Biochemical and Biophysical Research Communications 44:396–402
    [Google Scholar]
  15. McClary D. O., Bowers W. D. 1967; Structural differentiation of obligately aerobic and facultatively anaerobic yeasts. Journal of Cell Biology 32:519–524
    [Google Scholar]
  16. Mahler H. R., Perlman P. S. 1971; Mitochondriogenesis analysed by blocks on mitochondrial translation and transcription. Biochemistry 10:2979–2989
    [Google Scholar]
  17. Mahler H. R., Perlman P. S. 1972; Effects of mutagenic treatment by ethidium bromide on cellular and mitochondrial phenotype. Archives of Biochemistry and Biophysics 148:115–129
    [Google Scholar]
  18. Nagley P., Linnane A. W. 1970; Mitochondrial DNA deficient petite mutants of yeast. Biochemical and Biophysical Research Communications 39:989–996
    [Google Scholar]
  19. Perlman P. S., Mahler H. R. 1971a; A premutational state induced in yeast by ethidium bromides. Biochemical and Biophysical Research Communications 44:261–267
    [Google Scholar]
  20. Perlman P. S., Mahler H. R. 1971b; Molecular consequences of ethidium bromide mutagenesis. Nature, London 231:12–16
    [Google Scholar]
  21. Preer J. R. Jun 1971; Extrachromosomal inheritance: hereditary symbiants, mitochondria, chloroplasts. Annual Review of Genetics 5:361–406
    [Google Scholar]
  22. Slonimski P. P. 1956; Adaptation respiratoire: développement du système hémoproteique induit par l’oxygène. Proceedings of the 3rd International Congress of Biochemistry pp 242–252 New York: Academic Press;
    [Google Scholar]
  23. Slonimski P. P., Perrodin G., Croft J. H. 1968; Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal ‘petites’. Biochemical and Biophysical Research Communications 30:232–239
    [Google Scholar]
  24. Weislogel P. O., Butow R. A. 1971; Control of the mitochondrial genome in Saccharomyces cerevisiae. The fate of mitochondrial membrane proteins and mitochondrial deoxyribonucleic acid during petite induction. Journal of Biological Chemistry 246:5113–5119
    [Google Scholar]
  25. Wickerham L. J. 1970; Hansenula H. et P. Sydow. In The Yeasts: A Taxonomic Study pp 226–315 Edited by Lodder J. Amsterdam: North-Holland Publishing Co;
    [Google Scholar]
/content/journal/micro/10.1099/00221287-75-2-363
Loading
/content/journal/micro/10.1099/00221287-75-2-363
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error