RT Journal Article SR Electronic(1) A1 Preston, T. M. A1 O'Dell, D. S.YR 1973 T1 Deuterium Oxide-induced Reversion of Naegleria gruberi Flagellates JF Microbiology, VO 75 IS 2 SP 351 OP 361 DO https://doi.org/10.1099/00221287-75-2-351 PB Microbiology Society, SN 1465-2080, AB SUMMARY: A bead column technique is described for the separation of Naegleria gruberi flagellates from amoebae and cysts. It is shown that D2O induces a rapid reversion of these flagellates to amoebae, and the behaviour and structure of the reverting cells is described. The amoebae are viable in high concentrations of D2O, and when returned to suspension in H2O readily retransform to the flagellate stage. The reversion of flagellates to amoebae is insensitive to cycloheximide, but the subsequent retransformation to flagellate can be prevented by both actinomycin D and cycloheximide., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-75-2-351