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SUMMARY: A correlated electron microscopy and biochemical study of the effects of staphylococcal α-toxin on rabbit erythrocyte membrane structure has been carried out. Using several criteria, no evidence was obtained for the involvement of a proteolytic mechanism in the mode of action of this toxin.
After toxin treatment, ghosts examined by freeze-etching showed changes in micromorphology consistent with a hydrophobic interaction between toxin and membrane.
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