1887

Abstract

SUMMARY: A method is described for the production of dihydrogenated ergot alkaloids, principally dihydroergosine, in yields exceeding 0.5 mg/ml in surface liquid cultures of Alkaloid production occurred at the end of the growth phase and most of the product was released into the medium. An increase in the proportion of unsaturated fatty acids in the mycelial triglyceride oils during the growth phase was associated with the development of sclerotium-like tissues within the mycelial mat. Conversion of asparagine in the medium to aspartic acid by a non-alkaloid-producing strain indicated asparaginase activity. Inorganic phosphate was rapidly absorbed from the medium, and sucrose was utilized through hydrolysis followed by the preferential uptake of glucose. A glucan accumulated during growth of the alkaloid-producing strain, thus precluding a shake-culture process, whereas the small amount which was produced by a non-alkaloid-producing strain disappeared towards the end of the fermentation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-75-2-275
1973-04-01
2021-10-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/75/2/mic-75-2-275.html?itemId=/content/journal/micro/10.1099/00221287-75-2-275&mimeType=html&fmt=ahah

References

  1. Castagnoli N., Mantle P. G. 1966; Occurrence of d-lysergic acid and 6-methyl-ergol-8-ene-8-carboxylic acid in culture of Claviceps purpurea. Nature, London 211:859–860
    [Google Scholar]
  2. Dickerson A. G., Mantle P. G., Szczyrbak C. A. 1970; Autolysis of extracellular glucans produced in vitro by a strain of Claviceps fusiformis. Journal of General Microbiology 60:403–415
    [Google Scholar]
  3. Mantle P. G. 1968; Studies on Sphacelia sorghi McRae, an ergot of Sorghum vulgare Pers. Annals of Applied Biology 62:443–449
    [Google Scholar]
  4. Mantle P. G. 1972; Fatty-acid composition of triglyceride oils from sphacelial, sclerotial and stromatal tissues of Claviceps purpurea and Claviceps sulcata. Transactions of the British Mycological Society 59:325–327
    [Google Scholar]
  5. Mantle P. G., Finn C. A. 1971; Investigations on the mode of action of d-6-methyl-8-cyanomethyI-ergoline in suppressing pregnancy in the mouse. Journal of Reproduction and Fertility 24:441–444
    [Google Scholar]
  6. Mantle P. G., Morris L. J., Hall S. W. 1969; Fatty acid composition of sphacelial and sclerotial growth forms of Claviceps purpurea in relation to the production of ergoline alkaloids in culture. Transactions of the British Mycological Society 53:441–447
    [Google Scholar]
  7. Mantle P. G., Szczyrbak C. A. 1972; Factors affecting clavine alkaloid production in submerged cultures of Claviceps fusiformis. Journal of General Microbiology 73:xxii
    [Google Scholar]
  8. Mantle P. G., Tonolo A. 1968; Relationship between the morphology of Claviceps purpurea and the production of alkaloids. Transactions of the British Mycological Society 51:499–505
    [Google Scholar]
  9. Mantle P. G., Waight E. S. 1968; Dihydroergosine: a new naturally occurring alkaloid from sclerotia of Sphacelia sorghi McRae. Nature, London 218:581–582
    [Google Scholar]
  10. Rezabek K., Semonsky M., Kucharczyk N. 1969; Suppression of conception with d-6-methyl-8-cyanomethylergoline (I) in rats. Nature, London 221:666
    [Google Scholar]
  11. Thomas A. J. 1969 In Automation, Mechanisation and Data Handling in Microbiology Technical Series no. 4. Edited by Society for Applied Bacteriology London: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-75-2-275
Loading
/content/journal/micro/10.1099/00221287-75-2-275
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error