1887

Abstract

SUMMARY: Chicken antisera to the s6 strain of were used to study the mechanism of metabolic inhibition and growth inhibition by specific antibody. In high dilutions of antiserum the lag phase of growth was significantly prolonged, but eventually growth commenced and proceeded normally. In low dilutions of antiserum, growth was permanently inhibited. The prolongation of the lag phase was greater in a medium containing 10% horse serum than in 10% swine serum; with chicken serum in the medium the lag phase was of normal duration. The results correlated with the observation that the metabolic inhibition (m.i.) titres of antisera were higher when the test was conducted in horse serum than in swine or chicken serum. The antigens on the organism associated with the inhibition of growth by antiserum appeared to be essential physiologically active receptors. Antisera to growth medium caused agglutination of the organism, but did not inhibit growth or metabolism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-75-1-43
1973-03-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/75/1/mic-75-1-43.html?itemId=/content/journal/micro/10.1099/00221287-75-1-43&mimeType=html&fmt=ahah

References

  1. Adler H. E. 1954; A rapid slide agglutination test for the diagnosis of chronic respiratory disease in the field and in laboratory infected chickens and turkeys. Proceedings 90th Annual Meeting American Veterinary Association346
    [Google Scholar]
  2. Adler H. E. 1958; A PPLO slide agglutination test for the detection of infectious sinusitis in turkeys. Poultry Science 37:1116
    [Google Scholar]
  3. Adler H. E. 1967; The antiglobulin procedure for the detection of antibodies of avian origin. Avian Diseases 11:69–78
    [Google Scholar]
  4. Barker L. F., Platt J. K. 1967; Role of complement in immune inactivation of Mycoplasma gallisepticum. Journal of Bacteriology 94:403–408
    [Google Scholar]
  5. Jordan F. T. C., Kulasegaram P. 1968; Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum. Journal of Comparative Pathology 78:407–414
    [Google Scholar]
  6. McMartin D., Adler H. E. 1961; An immunological phenomenon in chickens following infection with Mycoplasma gallisepticum. Journal of Comparative Pathology and Therapeutics 71:311–323
    [Google Scholar]
  7. Purcell R. H., Wong D., Chanock R. M., Taylor-Robinson D., Canchola A., Valdesuso J. 1967; Significance of antibody to Mycoplasma as measured by metabolic-inhibition techniques. Annals of the New York Academy of Science 143:664–675
    [Google Scholar]
  8. Smith S. C., Dunlop W. R., Strout A. G. 1966; Effect of culture medium on antigenic structure of Mycoplasma. Avian Diseases 10:173–176
    [Google Scholar]
  9. Taylor-Robinson D., Berry D. M. 1969; The evaluation of the metabolic inhibition technique for the study of Mycoplasma gallisepticum. Journal of General Microbiology 55:127–137
    [Google Scholar]
  10. Warren J., Senterfit L. B., Siero F. 1968; Inactivated culture vaccine against Mycoplasma gallisepticum infection in chickens. American Journal of Veterinary Research 29:1659–1664
    [Google Scholar]
  11. Williams M. H., Taylor-Robinson D. 1967; Antigenicity of Mycoplasma membranes. Nature, London 215:973–974
    [Google Scholar]
  12. Zander D. V. 1961; Origin of s6 strain of Mycoplasma. Avian Diseases 5:154–156
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-75-1-43
Loading
/content/journal/micro/10.1099/00221287-75-1-43
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error