SUMMARY: The following requirements should be met by polyploid yeast sets optimally suited for gene dosage studies: isogenicity; homozygous wild-type genetic background; amenability to genetic analysis and preferably normal segregation of the mating type alleles; ploidy number as high as possible, but compatible with the above requirements.
The present study shows that tetraploid yeast sets which meet all these requirements may be developed with relative ease by the controlled cross procedure. The aa and αα diploids which constitute the parents of the crosses are obtained either by the selection of cells appearing spontaneously in haploid cultures upon prolonged cultivation (14 clones have thus been obtained), or by the choice of appropriate genotypes among the progeny of tetraploid clones.
This paper reports the segregation data of a typical complete genetic analysis in which tetraploid clones are reduced by two successive meioses to the haploid state; together with first meiosis segregation data from about seventy tetraploid clones.
BéchetJ.,
GrensonM.,
WiameJ. M.1970; Mutations affecting the repressibility of arginine biosynthetic enzymes in Saccharomyces cerevisiae. European Journal of Biochemistry 12:31–39
GrensonM.,
MoussetM.,
WiameJ. M.,
BéchetJ.1966; Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. Biochimica et biophysica acta 127:325–338
HennautC.,
HilgerF.,
GrensonM.1970; Space limitation for permease insertion in the cytoplasmic membrane of Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 39:666–671
HilgerF.,
CulotM.,
MinetM.,
PiérardA.,
GrensonM.,
WiameJ. M.1973; Studies on the kinetics of the enzyme sequence mediating arginine synthesis in Saccharomyces cerevisiae. Journal of General Microbiology 75:33–41
LacrouteF.,
PiérardA.,
GrensonM.,
WiameJ. M.1965; The biosynthesis of carbamoyl phosphate in Saccharomyces cerevisiae. Journal of General Microbiology 40:127–142
LaskowskiW.1960; Inaktivierungsversuche mit homozygoten Hefestämmen verschiedenen Ploidiegrades. I. Aufbau homozygoter Stämme und Dosiseffektkurven für ionisierende Strahlen, UV und organische Peroxyde. Zeitschrift für Naturforschung 156:495–506
LaskowskiW.1962; Inaktivierungsversuche mit homozygoten Hefestämmen verschiedenen Ploidiegrades. IV. Über den Aufbau weitestgehend isogener, homozygoter penta-und hexaploider Stämme sowie, den Einfluss bestimmter mutierter Allele auf die Strahlenresistenz. Zeitschrift für Naturforschung 176:93–188
MortimerR. K.1958; Radiobiological and genetic studies on a polyploid series (haploid to hexaploid) of Saccharomyces cerevisiae. Radiation Research 9:312–326
PomperS.,
BurkholderP. R.1949; Studies in the biochemical genetics of yeast. Proceedings of the National Academy of Sciences of the United States of America 35:456–464
ReichertU.1967; Gendosiswirkungen in einem ad2 Mutantensystem bei Saccharomyces. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene 205:63–68
RomanH.1962; Sources of variability in vegetative yeast cultures. Symposium held at the 8th International Congress of Microbiology, Montréal pp 306–312 University of Toronto Press;
RomanH.,
HawthorneD. C.,
DouglasH. C.1951; Polyploidy in yeast and its bearing on the occurrence of irregular genetic ratios. Proceedings of the National Academy of Sciences of the United States of America 37:79–84
RomanH.,
SandsS. M.1953; Heterogeneity of clones of Saccharomyces derived from haploid ascospores. Proceedings of the National Academy of Sciences of the United States of America 39:171–179
SchedaA.1963; Untersuchungen über die Maltose- und Glucosevergärung bei homozygoten Hefestäm-men mit verschiedenen Genomzahlen. Archiv für Mikrobiologie 45:65–100
SlonimskiP. P.,
ArcherR.,
PéréG.,
SelsA.,
SomloM.1965Mécanismes de régulation des activités cellulaires chez les microorganismes pp 435–461 Paris: Editions du Centre National de la Recherche Scientifique;
ThuriauxP.,
RamosF.,
WiameJ. M.,
GrensonM.,
BéchetJ.1968; Sur l’existence de gènes régulateurs affectant simultanément la synthése des enzymes biosynthétiques et cataboliques de l’arginine chez Saccharomyces cerevisiae. Archives internationales de physiologie et de biochimie 76:955
TownsendG. F.,
LindegrenC. C.1954; Characteristic growth patterns of the different members of a polyploid series of Saccharomyces. Journal of Bacteriology 67:480–483
WiameJ. M.1970; Mechanism of the interaction between the anabolism and catabolism of arginine in Saccharomyces cerevisiae. Report to the 10th International Congress of Microbiology, Mexico pp 243–253
WiameJ. M.1971; The regulation of arginine metabolism in Saccharomyces cerevisiae: exclusion mechanisms. In Current Topics in Cellular Regulation vol iv pp 1–38 Edited by
HoreckerB.,
StadtmanE.
New York: Academic Press;