SUMMARY: Growth of on methane was inhibited by methanol (0.1%, v/v, and above), ethanol, -propanol and -butanol (0.01%, v/v, and above), but was unaffected by galactose, glucose, fructose, maltose, sucrose (at 0.1 M) or lactose (0.05 M). About one organism in 7 million grew well on solid medium using methanol vapour as a sole source of carbon and energy, but [C]methanol was readily metabolized and assimilated by cultures growing on methane. Labelling patterns from [C]methane and [C]methanol were similar, indicating their assimilation by a common pathway. Dissimilarities between the labelling patterns obtained with CH and C-labelled formaldehyde, formate and carbonate indicated that the ribose phosphate cycle of formaldehyde assimilation may not account for all the carbon assimilated by significant incorporation of formate, carbon dioxide and possibly of intermediates of methane oxidation more reduced than formaldehyde may occur. C-Labelled ethanol and acetate showed restricted incorporation into lipid, leucine, glutamate, proline and arginine, indicating that can produce acetyl coenzyme A from both compounds and introduce it into an incomplete biosynthetic tricarboxylic acid cycle. was unable to assimilate more than trace amounts of [C]glucose or sucrose.


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