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Volume 75, Issue 1

Assimilation and Toxicity of Some Exogenous C Compounds, Alcohols, Sugars and Acetate in the Methane-oxidizing Bacterium Free

Abstract

SUMMARY: Growth of on methane was inhibited by methanol (0.1%, v/v, and above), ethanol, -propanol and -butanol (0.01%, v/v, and above), but was unaffected by galactose, glucose, fructose, maltose, sucrose (at 0.1 M) or lactose (0.05 M). About one organism in 7 million grew well on solid medium using methanol vapour as a sole source of carbon and energy, but [C]methanol was readily metabolized and assimilated by cultures growing on methane. Labelling patterns from [C]methane and [C]methanol were similar, indicating their assimilation by a common pathway. Dissimilarities between the labelling patterns obtained with CH and C-labelled formaldehyde, formate and carbonate indicated that the ribose phosphate cycle of formaldehyde assimilation may not account for all the carbon assimilated by significant incorporation of formate, carbon dioxide and possibly of intermediates of methane oxidation more reduced than formaldehyde may occur. C-Labelled ethanol and acetate showed restricted incorporation into lipid, leucine, glutamate, proline and arginine, indicating that can produce acetyl coenzyme A from both compounds and introduce it into an incomplete biosynthetic tricarboxylic acid cycle. was unable to assimilate more than trace amounts of [C]glucose or sucrose.

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© Society for General Microbiology, 1973
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1973-03-01
2023-05-29
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References

  1. Amemiya K. 1972; Absence of hexokinase in Methanomonas sp. as a possible cause of their inability to grow on glucose. Abstracts of the 72nd Annual Meeting of the American Society for Microbiology p 142
     [Google Scholar]
  2. Davey J. F., Whittenbury R., Wilkinson J. F. 1972; Distribution of key enzymes in the methane oxidizing bacteria. Archiv für Mikrobiologie 87:359–366
     [Google Scholar]
  3. Davies S. L., Whittenbury R. 1970; Fine structure of methane and other hydrocarbon-utilizing bacteria. Journal of General Microbiology 61:227–232
     [Google Scholar]
  4. Eccleston M., Kelly D. P. 1972a; Assimilation and toxicity of exogenous amino acids in the methane-oxidizing bacterium Methylococcus capsulatus. Journal of General Microbiology 71:541–554
     [Google Scholar]
  5. Eccleston M., Kelly D. P. 1972b; Competition among amino acids for incorporation into Methylococcus capsulatus. Journal of General Microbiology 73:303–314
     [Google Scholar]
  6. Eroshin V. K., Harwood J. H., Pirt S. J. 1968; Influence of amino acids, carboxylic acids and sugars on the growth of Methylococcus capsulatus on methane. Journal of Applied Bacteriology 31:560–567
     [Google Scholar]
  7. Foster J. W., Davis R. H. 1966; A methane-dependent coccus, with notes on classification and nomenclature of obligate, methane-utilizing bacteria. Journal of Bacteriology 91:1924–1931
     [Google Scholar]
  8. Hamer G., Heden C. G., Carenberg C. O. 1967; Methane as a carbon source for the production of microbial cells. Biotechnology and Bioengineering 9:499–514
     [Google Scholar]
  9. Higgins I. J., Quayle J. R. 1970; Oxygenation of methane by methane-grown Pseudomonas methanica and Methanomonas methanica. Biochemical Journal 118:201–208
     [Google Scholar]
  10. Johnson P. A., Quayle J. R. 1965; Microbial growth on C1 compounds. Synthesis of cell constituents by methane- and methanol-grown Pseudomonas methanica. Biochemical Journal 95:859–867
     [Google Scholar]
  11. Kelly D. P. 1970; Metabolism of organic acids by Thiobacillus neapolitanus. Archiv für Mikrobiologie 73:177–192
     [Google Scholar]
  12. Kelly D. P. 1971; Autotrophy: concepts of lithotrophic bacteria and their organic metabolism. Annual Review of Microbiology 25:177–210
     [Google Scholar]
  13. Kemp M. B., Quayle J. R. 1966; Microbial growth on C1 compounds. Incorporation of C1 units into allulose phosphate by extracts of Pseudomonas methanica. Biochemical Journal 99:41–48
     [Google Scholar]
  14. Kemp M. B., Quayle J. R. 1967; Microbial growth on C1 compounds. Uptake of [14C]formaldehyde and [14C]formate by methane-grown Pseudomonas methanica and determination of the hexose labelling pattern after brief incubation with [14C]methanol. Biochemical Journal 102:94–102
     [Google Scholar]
  15. Kirikova N. N., Romanova A. K. 1972; Assimilation of carbon dioxide by the cells of Pseudomonas sp. 2 utilizing methanol and formate. Mikrobiologiya 41:202–206
     [Google Scholar]
  16. Lawrence A. J., Kemp M. B., Quayle J. R. 1970; Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans. Biochemical Journal 116:631–639
     [Google Scholar]
  17. Lawrence A. J., Quayle J. R. 1970; Alternative Carbon Assimilation pathways in methane-utilizing bacteria. Journal of General Microbiology 63:371–374
     [Google Scholar]
  18. Namsaraev B. B., Nozhevnikova A. N., Zavarzin G. A. 1971; Utilization of formic acid by hydrogen bacteria. Mikrobiologiya 40:783–789
     [Google Scholar]
  19. Pan P., Umbreit W. W. 1972; Growth of obligate autotrophic bacteria on glucose in a continuous flow-through apparatus. Journal of Bacteriology 109:1149–1155
     [Google Scholar]
  20. Patel R. N., Bose H. R., Mandy W. J., Hoare D. S. 1972; Physiological studies of methane- and methanol-oxidizing bacteria: comparison of a primary alcohol dehydrogenase from Methylococcus capsulatus (Texas strain) and Pseudomonas species M27. Journal of Bacteriology 110:570–577
     [Google Scholar]
  21. Patel R. N., Hoare D. S. 1971; Physiological studies of methane and methanol-oxidizing bacteria: oxidation of C-1 compounds by Methylococcus capsulatus. Journal of Bacteriology 107:187–192
     [Google Scholar]
  22. Patel R., Hoare D. S., Taylor B. F. 1969; Biochemical basis for the obligate C-1 dependence of Methylococcus capsulatus. Bacteriological Proceedings128
     [Google Scholar]
  23. Quayle J. R. 1969; Microbial growth on C1 compounds. Process Biochemistry 4:25–29
     [Google Scholar]
  24. Quayle J. R. 1972; The metabolism of one-carbon compounds by microorganisms. Advances in Microbial Physiology 7:119–203
     [Google Scholar]
  25. Quayle J. R., Keech D. B. 1959; Carbon assimilation by Pseudomonas oxalaticus (OX1). Biochemical Journal 72:623–630631–637
     [Google Scholar]
  26. Ribbons D. W., Harrison J. E., Wadzinski A. M. 1970; Metabolism of single carbon compounds. Annual Review of Microbiology 24:135–158
     [Google Scholar]
  27. Ribbons D. W., Michalover J. L. 1970; Methane oxidation by cell-free extracts of Methylococcus capsulatus. FEBS Letters 11:41–44
     [Google Scholar]
  28. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. J., Britten R. J. 1955 Studies of Biosynthesis in Escherichia coli Publication of the Carnegie Institution 607 Washington, D.C.: Carnegie Institution;
     [Google Scholar]
  29. Shulgovskaya E. M., Andreeva E. A., Rabotnova I. L. 1971; Isolation and properties of the methanol-oxidizing bacterium Pseudomonas methanolica n.sp. Mikrobiologiya 40:862–865
     [Google Scholar]
  30. Silverman M. P. 1964 Methane-oxidizing Bacteria, a Review of the Literature Bureau of Mines Information Circular 8246 pp 37 Washington, D.C.: U. S. Department of the Interior;
     [Google Scholar]
  31. Stokes J. E., Hoare D. S. 1969; Reductive pentose cycle and formate assimilation in Rhodopseudomonas palustris. Journal of Bacteriology 100:890–894
     [Google Scholar]
  32. Wadzinski A. M., Ribbons D. W. 1972; Tricarboxylic acid cycle in the methylotroph, Methanomonas methanooxidans. Abstracts of the 72nd Annual Meeting of the American Society for Microbiology166
     [Google Scholar]
  33. Whittenbury R. 1969; Microbial utilization of methane. Process Biochemistry 4:51–56
     [Google Scholar]
  34. Whittenbury R. 1971; A speculative discussion of the relationship of the methane-oxidizing bacteria to chemolithotrophs. Journal of General Microbiology 69:v
     [Google Scholar]
  35. Whittenbury R., Phillips K. C., Wilkinson J. F. 1970; Enrichment, isolation and some properties of methane-utilizing bacteria. Journal of General Microbiology 61:205–218
     [Google Scholar]
  36. Wilkinson J. F. 1971; Hydrocarbons as a source of single cell protein. In Microbes and Biological Productivity. Symposium of the Society for General Microbiology 21:15–46
     [Google Scholar]
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Assimilation and Toxicity of Some Exogenous C1 Compounds, Alcohols, Sugars and Acetate in the Methane-oxidizing Bacterium Methylococcus capsulatus
Microbiology 75, 211 (1973); https://doi.org/10.1099/00221287-75-1-211
/content/journal/micro/10.1099/00221287-75-1-211
/content/journal/micro/10.1099/00221287-75-1-211
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