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SUMMARY: Wild-type strains of Pseudomonas putida, P. cepacia and P. acidovorans grew in minimal salts media with acetamide or phenylacetamide as the carbon or nitrogen source. Wild-type strains of P. aeruginosa utilized acetamide but not phenylacetamide. Pseudomonas putida A90 and mutants isolated from P. putida A87 and from P. cepacia 716 grew on phenylacetamide but not on acetamide. These activities were due to two types of amidase: (i) aliphatic amidases, which hydrolyse acetamide, produced by P. aeruginosa and strains of P. putida, P. cepacia and P. acidovorans; and (ii) phenylacetamidases, which hydrolyse phenylacetamide, produced by some strains of P. putida, P. cepacia and P. acidovorans.
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