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SUMMARY: Thirty-two culture collection strains of the thermophilic species of the genus Lactobacillus (Lactobacillus helveticus, L. jugurti, L. bulgaricus and L. lactis) and 13 field strains from natural whey cheese cultures were examined by routine physiological and serological tests. Genetic relatedness was determined by DNA–DNA molecular hybridization.
General agreement was observed between the phenotypic traits and genome homology. The close phenotypic resemblance between Lactobacillus helveticus and L. jugurti and between L. bulgaricus and L. lactis was confirmed by the genetic findings.
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