SUMMARY: Protein synthesis and translocation of l-[14C]leucine, d-[14C]glucose and inorganic 32P were enhanced in cultures of Sclerotium rolfsii Sacc. grown on medium supplemented with 0·5% lactose. Ethanol (2%, v/v) inhibited translocation but not protein synthesis. Neither lactose nor ethanol affected the uptake of radioactive substances by the fungal mycelium. Cycloheximide applied to the colony margins prevented sclerotium formation and protein synthesis without inhibiting translocation. It was concluded that translocation and protein synthesis in S. rolfsii are independent processes and are both essential for sclerotium formation.
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