1887

Abstract

SUMMARY: Crude cell-free amino acid-incorporating systems derived from a variety of enterobacterial species are not uniform in their ability to translate RNA of the coliphages Qβ or MS2. and to a lesser extent translate effectively under standard conditions, but and do not. Ineffective species do not generally inactivate phage RNA faster than effective ones, and they are as effective as in their general capacity for mRNA translation. This was judged in representative species by the incorporation of phenylalanine and leucine labels as directed by polyuridylate or mRNA of phage-infected , and from the relative incorporation of methionine label into the normal initiating -formyl-methionine residues of polypeptides. The ribosomal rather than the soluble components appear responsible for ineffectiveness in translation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-74-1-93
1973-01-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/74/1/mic-74-1-93.html?itemId=/content/journal/micro/10.1099/00221287-74-1-93&mimeType=html&fmt=ahah

References

  1. Adams J. M. 1968; On the release of the formyl group from nascent protein. Journal of Molecular Biology 33:571–569
    [Google Scholar]
  2. Bolle A., Epstein R. H., Salzer W. 1968; Transcription during bacteriophage T4 development: Synthesis and relative stability of early and late RNA. Journal of Molecular Biology 31:325–348
    [Google Scholar]
  3. Davis J. E., Sinsheimer R. L. 1963; The replication of bacteriophage MS 2. 1. Transfer of parental nucleic acid to progeny phage. Journal of Molecular Biology 6:203–207
    [Google Scholar]
  4. Dube S. K., Rudland P. S. 1970; Control of translation by T4 phage: altered binding of disfavoured messengers. Nature, London 231:820–823
    [Google Scholar]
  5. Haenni A. L., Chapeville F. 1966; The behaviour of acetylphenylalanyl soluble ribonucleic acid in polyphenylalanine synthesis. Biochimica et biophysica acta 114:135–148
    [Google Scholar]
  6. Hsu W. T., Weiss S. B. 1969; Selective translation of T4 template RNA by ribosomes from T4-infected Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 64:345–351
    [Google Scholar]
  7. Lodish H. F. 1970; Specificity in bacterial protein synthesis: role of initiation factors and ribosomal subunits. Nature, London 226:705–707
    [Google Scholar]
  8. Morrison T. G., Malamy M. H. 1971; T7 translational control mechanisms and their inhibition by F factors. Nature New Biology 231:37–41
    [Google Scholar]
  9. Nirenberg M. W. 1963; Cell-free protein synthesis directed by messenger RNA. Methods in Enzymology 6:17–27
    [Google Scholar]
  10. Pine M. J., Gordon B., Sarimo S. S. 1969; Protein initiation without folate in Streptococcus faecium. Biochimica et biophysica acta 179:439–447
    [Google Scholar]
  11. Sarimo S. S., Pine M. J. 1971; Evidence for the requirement of methionyl tRNA modification for in vitro polypeptide initiation in Streptococcus faecium. Biochimica et biophysica acta 240:233–236
    [Google Scholar]
  12. Schwartz J. H., Eisenstadt J. M., Brawerman G., Zinder N. D. 1965; Biosynthesis of the coat protein of coliphage f2 by extracts of Euglena gracilis. Proceedings of the National Academy of Sciences of the United States of America 53:195–200
    [Google Scholar]
  13. Szer W., Brenowitz J. 1970; Translation of MS 2 RNA by ribosomes from different bacterial species. Biochemical and Biophysical Research Communications 38:1154–1160
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-74-1-93
Loading
/content/journal/micro/10.1099/00221287-74-1-93
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error