SUMMARY: Crude cell-free amino acid-incorporating systems derived from a variety of enterobacterial species are not uniform in their ability to translate RNA of the coliphages Qβ or MS2. and to a lesser extent translate effectively under standard conditions, but and do not. Ineffective species do not generally inactivate phage RNA faster than effective ones, and they are as effective as in their general capacity for mRNA translation. This was judged in representative species by the incorporation of phenylalanine and leucine labels as directed by polyuridylate or mRNA of phage-infected , and from the relative incorporation of methionine label into the normal initiating -formyl-methionine residues of polypeptides. The ribosomal rather than the soluble components appear responsible for ineffectiveness in translation.


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