1887

Abstract

SUMMARY: Capsules of type III and type I were examined in the electron microscope using ruthenium red in combination with osmium tetroxide. The ability of this combination stain and fixative to stain polysaccharides has been well established. A slightly acid pH was necessary to obtain satisfactory capsular polysaccharide staining.

The capsules of both and were stained by the ruthenium red before alcohol dehydration and embedding in Epon 812. The capsule of had the appearance of a tightly woven mat. The capsule of had a fibrous appearance. Ruthenium red was found inside the plasma membrane of , but remained outside the cell membrane of .

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/content/journal/micro/10.1099/00221287-74-1-21
1973-01-01
2021-10-28
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