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SUMMARY: When Mycobacterium smegmatis was in an approximately balanced state of growth on glucose as the sole carbon source, free trehalose turned over at about 3 times the rate of its net formation, while the sugars of acyltrehalose and acylglucose turned over at about 13 and 8 times the rate of their net formation, respectively. Total lipid turned over substantially more slowly than this, while turnover of polysaccharide and of cell residue was slight or non-existent. However, in spite of their high turnover, the acylated sugars did not represent a major pathway of glucose metabolism, because of their low content in the bacteria.
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