%0 Journal Article %A Orpin, C. G. %T The Culture in vitro of the Rumen Bacterium Quin’s Oval %D 1972 %J Microbiology, %V 73 %N 3 %P 523-530 %@ 1465-2080 %R https://doi.org/10.1099/00221287-73-3-523 %I Microbiology Society, %X SUMMARY: The rumen bacterium Quin’s Oval has been maintained anaerobically in vitro for more than 1 year in liquid culture at 39°C, in the presence of other bacteria, rumen fluid, lactate, n-valerate, isovalerate, DL-α-methylbutyrate, L-cysteine, mannitol and a CO2 gas phase. The maximum population density attained was 8.1 × 106/ml. No growth was obtained in sterile media or in solid media. Growth and glycogen storage occurred when the organisms were incubated with mannitol, glucose, fructose, mannose and sucrose; amino acids, proteins and polysaccharides were not so utilized. The organisms did not survive freezing, but were viable after 2 weeks at 4°C. The morphology as seen in the light microscope and the staining of the organisms is described. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-73-3-523