RT Journal Article SR Electronic(1) A1 Coleman, G. S. A1 Davies, Judith I. A1 Cash, Margaret A.YR 1972 T1 The Cultivation of the Rumen Ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro JF Microbiology, VO 73 IS 3 SP 509 OP 521 DO https://doi.org/10.1099/00221287-73-3-509 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Populations of over 400 Epidinium ecaudatum caudatum/ml were maintained for over 18 months in the presence of bacteria, in a potassium phosphate-rich medium under 95% N2 + 5% CO2 with the daily addition of wholemeal flour and dried grass. Polyplastron multivesiculatum was also maintained at a population density of 45 to 70/ml under the same conditions, except that it was necessary to add each day a culture of another protozoon such as Epidinium ecaudatum caudatum, E. ecaudatum tricaudatum or Eudiplodinium maggii to give a population density of approximately 100/ml. The epidinia at least were engulfed by the polyplastron up to 10 being taken up each day; the minimum daily requirement for growth was one epidinium/polyplastron. Polyplastron multivesicutum also grew in the absence of other protozoa on a sodium chloride-rich medium, and if these protozoa were inoculated into the potassium phosphate-rich medium in the presence of epidinia, there was a lag of 3 to 5 days before they began to engulf the epidinia. The effect of other variations in the culture conditions is also described., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-73-3-509