1887

Abstract

SUMMARY: A wide diversity of treatments can cause rapid and extensive bursting of hyphal tips of Most hyphal tips from colonies grown on full-strength agar medium burst readily when flooded with distilled water. In contrast, hyphal tips from colonies grown on diluted medium survived flooding with distilled water but succumbed to dilute aqueous solutions (particularly acids, but also neutral salts, EDTA, alcohols, acetone, detergents). Apex bursting was generally inhibited by alkaline solutions but took place at certain concentrations of ethanolamine or NHOH. Mg, Mn or Ca caused pronounced swelling of the hyphal apex followed by an occasional burst. Sharp temperature increases also brought about apical disintegration regardless of nutrient concentration.

Colonies of sp., (wild-type and two morphological mutants), and Rhizopus arrhizus also underwent apical bursting when flooded with water or rapidly heated. , several spp., and some morphological mutants of were refractory.

The bursting of hyphal tips by flooding colonies with water is not simply an osmotic phenomenon; its temperature coefficient (Q 1.3 to 2.0) suggests the additional participation of biochemical reaction(s) as a rate-limiting step in the bursting process. Probably, enzymatic degradation and hence weakening of the wall is a prerequisite for bursting. These observations are offered as circumstantial evidence supporting the following conclusions: (i) the growing tips of fungi have a large wall lytic potential; (ii) the release of this activity during growth must be gradual and delicately co-ordinated with wall synthesis; (iii) this balance can be easily disturbed by a wide variety of external stimuli and the ensuing surge of lytic activity results in the violent disintegration of the hyphal apex.

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1972-12-01
2024-12-07
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References

  1. Bartnicki-Garcia S., Lippman E. 1966; Liberation of protoplasts from the mycelium of Phytophthora . Journal of General Microbiology 42:411–416
    [Google Scholar]
  2. Bartnicki-Garcia S., Lippman E. 1969; Fungal morphogenesis: Cell wall construction in Mucor rouxii . Science, Washington 165:302–304
    [Google Scholar]
  3. Bartnicki-Garcia S., Lippman E. 1972; Inhibition of Mucor rouxii by polyoxin D: effects on chitin synthetase and morphological development. Journal of General Microbiology 71:301–309
    [Google Scholar]
  4. Berger L. R., Weiser R. S. 1957; The β-glucosaminidase activity of egg-white lysozyme. Biochimica et biophysica acta 26:517–521
    [Google Scholar]
  5. Bracker C. E. 1971; Cytoplasmic vesicles in germinating spores of Gilbertella persicaria . Protoplasma 72:381–397
    [Google Scholar]
  6. Brenner D. M., Carroll G. C. 1968; Fine-structural correlates of growth in hyphae of Ascodesmis sphaerospora . Journal of Bacteriology 95:658–671
    [Google Scholar]
  7. Brody S. 1972; Metabolism, cell walls and morphogenesis. In Cell Differentiation. Edited by Coward S. New York: Academic Press; (In the press.)
    [Google Scholar]
  8. Endo A., Kakiki K., Misato T. 1970; Mechanism of action of the antifungal agent polyoxin D. Journal of Bacteriology 104:189–196
    [Google Scholar]
  9. Farkaš V., , Svoboda A., Bauer S. 1969; Inhibitory effect of 2-deoxy-D-glucose on the formation of the cell wall in yeast protoplasts. Journal of Bacteriology 98:744–748
    [Google Scholar]
  10. Gascón S., Ochoa A. G., Villanueva J. R. 1965; Production of yeast and mold protoplasts by treatment with the strepzyme of Micromonospora as. Canadian Journal of Microbiology 11:573–580
    [Google Scholar]
  11. Girbardt M. 1957; Der Spitzenkörper von Polystictus versicolor . Planta 50:47–59
    [Google Scholar]
  12. Girbardt M. 1969; Die Ultrastruktur der Apikalregion von Pilzhyphen. Protoplasma 67:413–441
    [Google Scholar]
  13. Gooday G. W. 1971; An autoradiographic study of hyphal growth of some fungi. Journal of General Microbiology 67:125–133
    [Google Scholar]
  14. Green P. B. 1969; Cell morphogenesis. Annual Review of Plant Physiology 20:365–394
    [Google Scholar]
  15. Grove S. N. 1971 Protoplasmic correlates of hyphal tip initiation and development in fungi. Ph.D. Thesis: Purdue University, Lafayette, Indiana, U.S.A.;
    [Google Scholar]
  16. Grove S. N., Bracker C. E. 1970; Protoplasmic organization of hyphal tips among fungi: vesicles and Spitzenkörper. Journal of Bacteriology 104:989–1009
    [Google Scholar]
  17. Grove S. N., Bracker C. E., Morré D. J. 1970; An ultrastructural basis for hyphal tip growth in Pythium ultimum . American Journal of Botany 57:245–266
    [Google Scholar]
  18. Johnson B. F. 1968; Lysis of yeast cell walls induced by 2-deoxyglucose at their sites of glucan synthesis. Journal of Bacteriology 95:1169–1172
    [Google Scholar]
  19. Katz D., Rosenberger R. F. 1971a; Hyphal wall synthesis in Aspergillus nidulans: effect of protein synthesis inhibition and osmotic shock on chitin insertion and morphogenesis. Journal of Bacteriology 108:184–190
    [Google Scholar]
  20. Katz D., Rosenberger R. F. 1971b; Lysis of an Aspergillus nidulans mutant blocked in chitin synthesis and its relation to wall assembly and wall metabolism. Archiv für Mikrobiologie 80:284–292
    [Google Scholar]
  21. McClure W. K., Park D., Robinson P. M. 1968; Apical organization in the somatic hyphae of fungi. Journal of General Microbiology 50:177–182
    [Google Scholar]
  22. McMurrough I., Bartnicki-Garcia S. 1971; Properties of a particulate chitin synthetase from Mucor rouxii . Journal of Biological Chemistry 246:4008–4016
    [Google Scholar]
  23. McMurrough I., Flores-Carreón A., Bartnicki-Garcia S. 1971; Pathway of chitin synthesis and cellular localization of chitin synthetase in Mucor rouxii . Journal of Biological Chemistry 246:3999–4007
    [Google Scholar]
  24. Mahadevan P. R., Mahadkar U. R. 1970; Role of enzymes in growth and morphology of Neurospora crassa: cell-wall-bound enzymes and their possible role in branching. Journal of Bacteriology 101:941–947
    [Google Scholar]
  25. Mahadevan P. R., Rao S. R. 1970; Enzyme degradation of conidial wall during germination of Neurospora crassa . Indian Journal of Experimental Biology 8:293–297
    [Google Scholar]
  26. Matile P., Cortat M., Wiemken A., Frey-Wyssling A. 1971; Isolation of glucanase-containing particles from budding Saccharomyces cerevisiae . Proceedings of the National Academy of Sciences of the United States of America 68:636–640
    [Google Scholar]
  27. Megnet R. 1965; Effect of 2-deoxyglucose on Schizosaccharomyces pombe . Journal of Bacteriology 90:1032–1035
    [Google Scholar]
  28. Mishra N. C., Tatum E. L. 1972; Effect of l-sorbose on polysaccharide synthetases of Neurospora crassa . Proceedings of the National Academy of Sciences of the United States of America 69:313–317
    [Google Scholar]
  29. Nickerson W. J., Falcone G. 1956; Identification of protein disulfide reductase as a cellular division enzyme in yeasts. Science, Washington 124:722–723
    [Google Scholar]
  30. Otakara A. 1961; Studies on the chitinolytic enzymes of black-koji mold. III. Liquefying activity and saccharifying activity of the chitinase preparation. Agricultural and Biological Chemistry 25:494–499
    [Google Scholar]
  31. Park D., Robinson P. M. 1966a; Internal pressure of hyphal tips of fungi, and its significance in morphogenesis. Annals of Botany 30:425–439
    [Google Scholar]
  32. Park D., Robinson P. M. 1966b; Aspects of hyphal morphogenesis in fungi. In Trends in Plant Morphogenesis, pp. 27–44 Edited by Cutter E. G. London: Longmans, Green & Co.;
    [Google Scholar]
  33. Reinhardt M. O. 1892; Das Wachstum der Pilzhyphen. Jahrbuch für wissenschaftliche Botanik 23:479–566
    [Google Scholar]
  34. Rizvi S. R. H., Robertson N. F. 1965; Apical disintegration of hyphae of Neurospora crassa as a response to l-sorbose. Transactions of the British Mycological Society 48:469–477
    [Google Scholar]
  35. Robertson N. F. 1958; Observations on the effect of water on the hyphal apices of Fusarium oxysporum . Annals of Botany 22:159–173
    [Google Scholar]
  36. Robertson N. F. 1959; Experimental control of hyphal branching and branch form in hyphomycetous fungi. Journal of the Linnean Society 56:207–211
    [Google Scholar]
  37. Robertson N. F. 1965; The fungal hypha. Transactions of the British Mycological Society 48:18
    [Google Scholar]
  38. Smith J. H. 1923; On the apical growth of fungal hyphae. Annals of Botany 37:341–343
    [Google Scholar]
  39. Strunk C. 1969; Licht- und elektronenmikroskopische Untersuchungen an jungen Protoplasten von Polystictus versicolor . Zeitschrift für allgemeine Mikrobiologie 9:49–60
    [Google Scholar]
  40. Tracey M. V. 1955; Chitinase in some Basidiomycetes. Biochemical Journal 61:579–586
    [Google Scholar]
  41. Wang M. C., Bartnicki- Garcia S. 1966; Biosynthesis of β-1, 3 and β-1,6-linked glucan by Phytophthora cinnamomi hyphal walls. Biochemical and Biophysical Research Communications 24:832–837
    [Google Scholar]
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