1887

Abstract

SUMMARY: The extracellular polygalacturonate lyase of a Bacillus species isolated from water-stored Sitka spruce () was purified by ammonium sulphate fractionation and CM-cellulose chromatography. Its specific activity increased 59-fold and amylase, protease and xylanase activities were removed.

Degradation of the substrate was accompanied by a large decrease in viscosity, suggesting random attack. While calcium and strontium ions activated the enzyme, most divalent cations caused inhibition. Addition of EDTA resulted in complete inactivation. Enzyme activity was higher with acid soluble pectic acid than with sodium polypectate. The purified enzyme was stable over a wide pH range and had considerable resistance to thermal inactivation. This stability explains the enzyme's prolonged activity in water-stored spruce sapwood.

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1972-12-01
2021-07-27
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References

  1. Bernfeld P. 1955; Amylases α and β . Methods in Enzymology 1:149–158
    [Google Scholar]
  2. Campbell L. L. 1955; Purification and properties of an α-amylase from facultative thermophilic bacteria. Archives of Biochemistry and Biophysics 54:154–161
    [Google Scholar]
  3. Dunleavy J. A., Fogarty W. M. 1971a; The preservation of spruce poles using a biological pretreatment. Proceedings of the British Wood Preserving Association 21: st Annual Convention; Cambridge: pp 1–24
    [Google Scholar]
  4. Dunleavy J. A., Fogarty W. M. 1971b; The effects of ponding on the permeability of Sitka spruce (Picea sitchensis) . Abstracts of 2nd International Biodeterioration Symposium, Lunteren, The Netherlands, 16:10
    [Google Scholar]
  5. Fogarty W. M., Ward O. P. 1972a; Enzyme production by bacteria isolated from water stored Sitka spruce (Picea sitchensis) . Journal of Applied Bacteriology (in the Press)
    [Google Scholar]
  6. Fogarty W. M., Ward O. P. 1972b; Microbial enzymes associated with increased permeability of Sitka spruce (Picea sitchensis) to preservatives: their detection in the expressed sap. Journal of General Microbiology 71:xi–xii
    [Google Scholar]
  7. Fuchs A. 1965; The trans-eliminative breakdown of Na-polygalacturonate by Pseudomonas fluorescens . Antonie Van Leeuwenhoek 31:323–340
    [Google Scholar]
  8. Garibaldi A., Bateman D. F. 1971; Pectic enzymes produced by Erwinia chrysanthemi and their effects on plant tissue. Physiological Plant Pathology 1:25–40
    [Google Scholar]
  9. Hasegawa S., Nagel C. W. 1966; A new pectic transeliminase produced exocellularly by a bacillus. Journal of Food Science 31:838–845
    [Google Scholar]
  10. Liese W. 1970; Ultrastructural aspects of woody tissue disintegration. Annual Review of Phytopathology 8:231–258
    [Google Scholar]
  11. Lowry O., Rosebrough N., Farr A., Randall R. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  12. McCready R. M., Seegmiller C. G. 1954; Action of pectic enzymes on oligogalacturonic acids and some of their derivatives. Archives of Biochemistry and Biophysics 50:440–450
    [Google Scholar]
  13. Macmillan J. D., Phaff H. J., Vaughn R. H. 1964; The pattern of action of an exopolygalacturonic acid-transeliminase from Clostridium multifermentans . Biochemistry 3:572–578
    [Google Scholar]
  14. Macmillan J. D., Vaughn R. H. 1964; Purification and properties of a polygalacturonic acid-transeliminase produced by Clostridium multifermentans . Biochemistry 3:564–572
    [Google Scholar]
  15. Moran F., Nasuno S., Starr M. P. 1968; Extracellular and intracellular polygalacturonic acid transeliminases of Erwinia carotovora . Archives of Biochemistry and Biophysics 123:298–306
    [Google Scholar]
  16. Moran F., Starr M. P. 1969; Metabolic regulation of polygalacturonic acid transeliminase in Erwinia . European Journal of Biochemistry 11:291–295
    [Google Scholar]
  17. Nagel C. W., Vaughn R. H. 1961; The characteristics of a polygalacturonase produced by Bacillus polymyxa . Archives of Biochemistry and Biophysics 93:344–352
    [Google Scholar]
  18. Nagel C. W., Wilson T. M. 1970; Pectic acid lyases of Bacillus polymyxa . Applied Microbiology 20:374–383
    [Google Scholar]
  19. Nasuno S., Starr M. P. 1967; Polygalacturonic acid transeliminase of Xanthomonas campestris . Biochemical Journal 104:178–185
    [Google Scholar]
  20. Okamoto K., Hatanaka C., Ozawa J. 1964; The mechanism of action of the saccharifying pectate transeliminase. Berichte des Ohara Instituts Fuer Landwirtschaftliche Biologie, Okayama Universitaet, Japan 12:115–119
    [Google Scholar]
  21. Spectrophotometry nomenclature 1965 Analytical Chemistry 37:1814
    [Google Scholar]
  22. Voragen A. G. J., Pilnik W. 1970; Pektindepolymerasen: I Einteilong der Pektinsauredepolymerasen Nach Ihrer Wirkung auf Oligomere Galakturonsauren. Zeitschrift für Lebensmittel-untersuchung und Forschung 124:346–359
    [Google Scholar]
  23. Ward O. P., Fogarty W. M. 1971; Extracellular enzymes of a Bacillus sp. associated with increased permeability in Sitka spruce (Picea sitchensis) . Biochemical Journal 125:109P–110P
    [Google Scholar]
  24. Zucker M., Hankin L. 1970; Regulation of pectate lyase synthesis in Pseudomonas fluorescens and Erwinia carotovora . Journal of Bacteriology 104:13–18
    [Google Scholar]
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