1887

Abstract

SUMMARY: Four enzymes (threonine deaminase, -acetohydroxyacid synthetase, iso-propylmalate synthetase and transaminase B) involved in leucine, isoleucine and valine synthesis have been detected in extracts of the blue-green alga and the control of three of the enzymes examined. A new procedure for the determination of transaminase B is described. Threonine deaminase and -acetohydroxyacid synthetase are subject to feedback control by their end-products. No evidence for the repression of enzyme synthesis in the pathway has been found, although the assimilation and incorporation of exogenous valine and leucine was demonstrated.

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1972-12-01
2024-04-19
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References

  1. Barritt G. J. 1971; Biosynthesis of isoleucine and valine in Rhodopseudomonas spheroides. Regulation of threonine deaminase activity. Journal of Bacteriology 105:718–721
    [Google Scholar]
  2. Black S., Wright N. G. 1955; β-Aspartokinase and β-aspartyl phosphate. Journal of Biological Chemistry 213:27–38
    [Google Scholar]
  3. Burns R. O., Calvo J., Margolin P., Umbarger H. E. 1966; Expression of the leucine operon. Journal of Bacteriology 91:1570–1576
    [Google Scholar]
  4. Bussey H., Umbarger H. E. 1969; Biosynthesis of branched-chain amino acids in yeast: Regulation of synthesis of the enzymes of isoleucine and valine biosynthesis. Journal of Bacteriology 98:623–628
    [Google Scholar]
  5. Calvo R., Calvo J. M. 1967; Lack of end-product inhibition and repression of leucine synthesis in a strain of Salmonella typhimurium . Science, New York 156:1107–1109
    [Google Scholar]
  6. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12. Journal of Bacteriology 60:17–28
    [Google Scholar]
  7. Dwyer S. B., Umbarger H. E. 1968; Isoleucine and valine metabolism in Escherichia coli XVI. Pattern of multivalent repression in strain k-12. Journal of Bacteriology 95:1680–1684
    [Google Scholar]
  8. Freundlich M., Burns O. R., Umbarger H. E. 1962; Control of isoleucine, valine and leucine biosynthesis. I. Multivalent repression. Proccedings of the National Academy of Sciences of the United States of America 48:1804–1808
    [Google Scholar]
  9. Friedemann T. E., Haugen G. E. 1943; Pyruvic acid. II. The determination of keto acids in blood and urine. Journal of Biological Chemistry 147:415–442
    [Google Scholar]
  10. Grunert R. R., Phillips P. H. 1951; A modification of the nitroprusside method of analysis for glutathione. Archives of Biochemistry 30:217–225
    [Google Scholar]
  11. Halpern Y. S., Umbarger H. E. 1959; Evidence for two distinct enzyme systems forming acetolactate in Aerobacter aerogenes . Journal of Biological Chemistry 234:3067–3071
    [Google Scholar]
  12. Hoare D. S., Hoare S. L., Moore R. B. 1967; The photo-assimilation of organic compounds by autotrophic blue-green algae. Journal of General Microbiology 49:351–370
    [Google Scholar]
  13. Hood W., Carr N. G. 1968; Threonine deaminase and acetolactate synthetase in Anabaena variabilis . Biochemical Journal 109:4 p.
    [Google Scholar]
  14. Hood W., Carr N. G. 1971; Apparent lack of control by repression of arginine metabolism in blue-green algae. Journal of Bacteriology 107:365–367
    [Google Scholar]
  15. Hood W., Leaver A. G., Carr N. G. 1969; Extracellular nitrogen and the control of arginine biosynthesis in Anabaena variabilis . Biochemical Journal 114:12–13 p.
    [Google Scholar]
  16. Kratz W. A., Myers J. 1955; Nutrition and growth of several blue-green algae. American Journal of Botany 42:282–287
    [Google Scholar]
  17. Leavitt R. I., Umbarger H. E. 1962; Isoleucine and valine metabolism in Escherichia coli. XI. Valine inhibition of the growth of Escherichia coli strain k-12. Journal of Bacteriology 83:624–630
    [Google Scholar]
  18. Lipmann F., Tuttle C. L. 1945; A specific micromethod for the determination of acyl phosphates. Journal of Biological Chemistry 159:21–28
    [Google Scholar]
  19. Magee P. T., Hereford L. M. 1969; Multivalent repression of isoleucine-valine biosynthesis in Saccharomyces cerevisiae . Journal of Bacteriology 98:857–862
    [Google Scholar]
  20. Meister A. 1957 Biochemistry of Amino Acids, p. 485 New York: Academic Press;
    [Google Scholar]
  21. Pearce J., Carr N. G. 1967; The metabolism of acetate by the blue-green algae, Anabaena variabilis and Anacystis nidulans . Journal of General Microbiology 49:301–313
    [Google Scholar]
  22. Pearce J., Carr N. G. 1969; The incorporation and metabolism of glucose by Anabaena variabilis . Journal of General Microbiology 54:451–462
    [Google Scholar]
  23. Pearce J., Leach C. K., Carr N. G. 1969; The incomplete tricarboxylic acid cycle in the blue-green alga, Anabaena variabilis . Journal of General Microbiology 55:371–378
    [Google Scholar]
  24. Radhakrishnan A. N., Snell E. E. 1960; Biosynthesis of valine and isoleucine. II. Formation of α-acetolactate and α-aceto-2-hydroxglutyrate in Neurospora crassa and Escherichia coli . Journal of Biological Chemistry 235:2316–2321
    [Google Scholar]
  25. Roberts R. B., Cowie D. B., Abelson P. H., Bolton E. T., Britten R. J. 1955 Studies of Biosynthesis in Escherichia coli, p. 521 Carnegie Institute of Washington Publication 607 Washington: Carnegie Institute;
    [Google Scholar]
  26. Umbarger H. E. 1969; Regulation of amino acid metabolism. Annual Review of Biochemistry 38:323–370
    [Google Scholar]
  27. Weber H. L., Böck A. 1968; Comparative studies on the regulation of DAHP synthetase activity in blue-green and green algae. Archiv für Mikrobiologie 61:159–168
    [Google Scholar]
  28. Westerfeld W. W. 1945; A colorimetric determination of blood acetoin. Journal of Biological Chemistry 161:495–502
    [Google Scholar]
  29. Yanofsky C., Reissig J. L. 1953; l-Serine dehydrase of Neurospora . Journal of Biological Chemistry 202:567–577
    [Google Scholar]
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