@article{mbs:/content/journal/micro/10.1099/00221287-73-2-395, author = "Ellwood, D. C. and Tempest, D. W.", title = "Influence of Culture pH on the Content and Composition of Teichoic Acids in the Walls of Bacillus subtilis", journal= "Microbiology", year = "1972", volume = "73", number = "2", pages = "395-402", doi = "https://doi.org/10.1099/00221287-73-2-395", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-73-2-395", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Teichoic acids were invariably present in the walls of Bacillus subtilis var. niger and B. subtilis W23 grown in chemostats at D = 0.25 h−1, 35 °C, pH 7.0 in media containing non-limiting concentrations of phosphate, but they possessed little ester-bound alanine. However, varying the culture pH between 8.0 and 5.0 effected changes not only in the amount but also in the ester-bound alanine content of wall teichoic acids of both organisms. The walls of phosphate-limited B. subtilis var. niger were devoid of teichoic acid when grown at pH 7.0, but not at lower pH, when this polymer again contained ester-bound alanine. The magnesium binding properties of these wall preparations were also determined. The results showed that walls containing ester-bound alanine in the teichoic acid bound the same amount of magnesium as walls in which the teichoic acid was unsubstituted by alanine.", }