1887

Abstract

SUMMARY: Studies have been carried out on the molecular nature of the plasmid DNA in four strains of carrying different mutants of the K88 factor. The factor was derepressed in three of the strains, one of which had kept the K88 antigen determinant, while the antigen was no longer demonstrable in the two other strains. The fourth strain possessed the K88 antigen but was transfer defective. The purified plasmid DNA was studied by electron microscopy and analytical CsCl density gradient centrifugation.

All the plasmid DNA from the four strains formed a single band in the neutral CsCl gradient at a density of 1.709 g/cm. The distributions of the contour lengths of the circular DNA molecules indicate three principal size classes of about 5, 20 and 25 µm. The small, 5 µm circles presumably contain the K88 determinant. The results seem to support the theory of a dissociation of composite plasmids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-73-2-373
1972-11-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/73/2/mic-73-2-373.html?itemId=/content/journal/micro/10.1099/00221287-73-2-373&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1950; Methods of study of bacterial viruses. Methods in Medical Research 2:1–73
    [Google Scholar]
  2. Anderson E. S. 1968; The ecology of transferable drug resistance in the enterobacteria. Annual Review of Microbiology 22:131–180
    [Google Scholar]
  3. Bak A. L., Christiansen c., Christiansen G. 1972; Circular, repetitive DNA in yeast. Biochimica et biophysica acta 267:527–530
    [Google Scholar]
  4. Bazaral m., Helinski D. R. 1968; Circular DNA forms of colicinogenic factors E1, E2, E3 from Escherichia coli . Journal of Molecular Biology 37:185–194
    [Google Scholar]
  5. Beckwith J. 1967; Regulation of the lac operon. Science, New York 156:597–604
    [Google Scholar]
  6. Cohen s. n., Miller C. A. 1969; Multiple molecular species of circular R–factor DNA isolated from Escherichia coli . Nature, London 224:1273–1277
    [Google Scholar]
  7. Cohen S. N., Miller C. A. 1970a; Non–chromosomal antibiotic resistance in bacteria. II. Molecular nature of R–factors isolated from Proteus mirabilis and Escherichia coli . Journal of Molecular Biology 50:671–687
    [Google Scholar]
  8. Cohen s. n., Miller C. A. 1970b; Non–chromosomal antibiotic resistance in bacteria. III. Isolation of the discrete transfer unit of the R–factor R1. Proceedings of the National Academy of the United States of America 67:510–516
    [Google Scholar]
  9. Davis J. E., Strauss J. H., Sinsheimer R. L. Jun 1961; Bacteriophage ms2: another RNA phage. Science, New York 134:1427
    [Google Scholar]
  10. Dettori R., Maccacaro G. A., Piccini G. L. 1961; Sex–specific bacteriophages of Escherichia coli K12. Giornale di microbiologia 9:141–150
    [Google Scholar]
  11. Goebel W., Schrempf H. 1971; Isolation and characterization of supercoiled circular deoxyribonucleic acid from beta–hemolytic strains of Escherichia coli . Journal of Bacteriology 106:311–317
    [Google Scholar]
  12. Haapala D. K., Falkow S. 1971; Physical studies of the drug–resistance transfer factor in Proteus . Journal of Bacteriology 106:294–295
    [Google Scholar]
  13. Hayes W. 1969; What are episomes and plasmids ?. In Bacterial Episomes and Plasmids, pp. 4–11 Edited by Wolstenholme G. E. W., O’Connor M. London: J. A. Churchill;
    [Google Scholar]
  14. Kontomichalou P., Mitani M., Clowes R. C. 1970; Circular R–factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control. Journal of Bacteriology 104:34–44
    [Google Scholar]
  15. Lederberg J. 1947; Gene recombination and linked segregation in Escherichia coli . Genetics 32:505–525
    [Google Scholar]
  16. Loeb T., Zinder N. D. 1961; A bacteriophage containing RNA. Proceedings of the National Academy of Sciences of the United States of America 47:282–289
    [Google Scholar]
  17. Meynell G. G. 1969 In Bacterial Episomes and Plasmids, p. 175 Edited by Wolstenholme G. E. W., O’Connor M. London: J. A. Churchill;
    [Google Scholar]
  18. Meynell e., Cooke M. 1969; Repressor–minus and operator-constitutive de–repressed mutants of F-like R factors; their effect on chromosomal transfer by hfrc. Genetical Research 14:309–313
    [Google Scholar]
  19. Meynell E., Datta N. 1966; The relation of resistance transfer factors to the F-factor (sex-factor) of Eschericha coli k12. Genetical Research 7:134–140
    [Google Scholar]
  20. Meynell E., Meynell G. G., Datta N. 1968; Phylogenetic relationships of drug–resistance factors and other transmissible bacterial plasmids. Bacteriological Reviews 32:55–83
    [Google Scholar]
  21. Nisioka T., Mitani M., Clowes R. C. 1969; Composite circular forms of R factor deoxyribonucleic acid molecules. Journal of Bacteriology 97:376–385
    [Google Scholar]
  22. Nisioka T., Mitani M., Clowes R. C. 1970; Molecular recombination bteween R-factor deoxyribonucleic acid molecules in Escherichia coli host cells. Journal of Bacteriology 103:166–177
    [Google Scholar]
  23. Ørskov i., Ørskov F. 1960; An antigen termed f + occurring in F+ E. coli strains. Acta pathologica et microbiologica scandinavica 48:37–46
    [Google Scholar]
  24. Ørskov I., Ørskov F. 1966; Episome–carried surface antigen K88 of Escherichia coli. I. Transmission of the determinant of the K88 antigen and influence on the transfer of chromosomal markers. Journal of Bacteriology 91:69–75
    [Google Scholar]
  25. Ørskov I., Ørskov F., Sojka W. J., Leach J. M. 1961; Simultaneous occurrence of E coli b and l antigens in strains from diseased swine. Acta pathologica et microbiologica scandinavica 53:404–422
    [Google Scholar]
  26. Ørskov I., Ørskov F., Sojka W. J., Wittig W. 1964; K antigens K88ab(L) and K88ac(L) in E. coli . Acta pathologica et microbiologica scandinavica 62:439–447
    [Google Scholar]
  27. Radloff R., Bauer W., Vinograd J. 1967; A dye–buoyant–density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 57:1514–1521
    [Google Scholar]
  28. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. Journal of Molecular Biology 4:430–443
    [Google Scholar]
  29. Silver R. P., Falkow S. 1970a; Specific labeling and physical characterization of R–factor deoxyribonucleic acid in Escherichia coli . Journal of Bacteriology 104:331–339
    [Google Scholar]
  30. Silver R. P., Falkow S. 1970b; Studies on resistance transfer factor deoxyribonucleic acid in Escherichia coli . Journal of Bacteriology 104:340–344
    [Google Scholar]
  31. Silverman P. M., Mobach H. W., Valentine R. C. 1967; Sex hair (F-pili) mutants of E. coli . Bio–chemical and Biophysical Research Communications 27:412–416
    [Google Scholar]
  32. Stirm S., Ørskov F., Ørskov I., Birch–Andersen A. 1967a; Episome–carried surface antigen K88 of Escherichia coli. III. Morphology. Journal of Bacteriology 93:740–748
    [Google Scholar]
  33. Stirm S., Ørskov F., Ørskov I., Mansa B. 1967b; Episome–carried surface antigen K88 of Escherichia coli. II. Isolation and chemical analysis. Journal of Bacteriology 93:731–739
    [Google Scholar]
  34. Vinograd J., Lebowitz J. 1966; Physical and topological properties of circular DNA. Journal of General Physiology 49:103–125
    [Google Scholar]
  35. Watanabe T. 1969; Transferable drug resistance: the nature of the problem. In Bacterial Episomes and Plasmids, pp. 81–97 Edited by Wolstenholme G. E.W., O’Connor M. London: J. A. Churchill;
    [Google Scholar]
  36. Watanabe T., Fukasawa T. 1961; Episome–mediated transfer of drug resistance in Enterobacteriaceae. Journal of Bacteriology 5:669–678
    [Google Scholar]
  37. Wohlhieter J. A., Falkow S., Citarella R. V. 1966; Purification of episomal DNA with cellulose nitrate membrane filters. Biochimica et biophysica acta 129:475–481
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-73-2-373
Loading
/content/journal/micro/10.1099/00221287-73-2-373
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error