SUMMARY: Polyribosomes were isolated from vegetative cells of NEFF. Ribosomes, of sedimentation coefficient 97 S, were isolated from vegetative cells and cysts. Both polyribosomes and ribosomes dissociated in 0.3 M-KC1 to particles of sedimentation coefficients 66, 53 and 40 S, and a slowly sedimenting particle whose sedimentation coefficient was not measured. RNA was extracted from the particles and fractionated. The 53 S and 40 S particles are the ribosomal subunits; the 97 and 66 S particles contained RNA characteristic of both subunits.


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