1887

Abstract

SUMMARY: sp. strain IV-85, an isolate from the marine environment, oxidized thiosulphate in two sequential logarithmic phases. Tetrathionate accumulated in the partially spent broth during the first logarithmic phase, and reached a maximum concentration at the time of transition to the second logarithmic phase. Tetrathionate added during the early first logarithmic phase caused a decrease in the rate of thiosulphate utilization similar to that observed after the transition from the first to second logarithmic phase. Bacteria harvested from the first logarithmic phase resisted 0.1 mM-cyanide which inhibited oxygen uptake by bacteria harvested from the second logarithmic phase. Rhodanese (thiosulphate: cyanide sulphur transferase, EC. 2.8.1.1) was detected in extracts of bacteria from both phases. Tetrathionate accumulation in the growth medium inhibited rhodanese activity, resulting in the transition of thiosulphate utilization from the first to second logarithmic phase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-73-2-339
1972-11-01
2022-05-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/73/2/mic-73-2-339.html?itemId=/content/journal/micro/10.1099/00221287-73-2-339&mimeType=html&fmt=ahah

References

  1. Bowen T. J., Butler P. J., Happold F. C. 1965; Some properties of the rhodanese system of Thiobacillus denitrificans . Biochemical Journal 97:651–657
    [Google Scholar]
  2. FIutchinson M., Johnstone K. I., White D. 1969; Taxonomy of the genus Thiobacillus: the outcome of numerical taxonomy to the group as a whole. Journal of General Microbiology 57:397–410
    [Google Scholar]
  3. Lang K. 1933; Die Rhodanbildung im Tierkörper. Biochemische Zeitschrift 259:243–256
    [Google Scholar]
  4. London J., Rittenberg S. C. 1964; Path of sulphur in sulphide and thiosulphate oxidation by thio- bacilli. Proceedings of the National Academy of Sciences of the United States of America 52:1183–1190
    [Google Scholar]
  5. Lowry O. H., Rosebrough N. J., Farr A. L, Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  6. Murphy J. R., Kornfeld J. M., Tilton R. C. 1971; Biphasic thiosulphate utilization by a marine Thiobacillus . Journal of General Microbiology 68:231–233
    [Google Scholar]
  7. Okuzumi M., Kita T. 1965; Studies on the biochemistry of the thiobacilli. VI. Oxidation of thiosulphate to tetrathionate by T. thiooxidans . Agricultural and Biological Chemistry 29:1063–1068
    [Google Scholar]
  8. Parker C. D., Prisk J. 1953; The oxidation of inorganic compounds of sulphur by various sulphur bacteria. Journal of General Microbiology 8:344–364
    [Google Scholar]
  9. Santer M., Margulies M., Klinman N., Kaback R. 1960; Role of inorganic phosphate in thiosulphate metabolism by Thiobacillus thioparus . Journal of Bacteriology 79:313–320
    [Google Scholar]
  10. Smith A. J., Lascelles J. 1966; Thiosulphate metabolism and rhodanese in Chromatium sp. strain d. Journal of General Microbiology 42:357–370
    [Google Scholar]
  11. Starkey R. L. 1934; The production of polythionates from thiosulphate by microorganisms. Journal of Bacteriology 28:387–400
    [Google Scholar]
  12. Tano T., Asano H., Imai K. 1968; Physiological studies on thiobacilli. III. Oxidation of thiosulphate by particulate fraction of Thiobacillus thiooxidans . Agricultural and Biological Chemistry 32:140–143
    [Google Scholar]
  13. Trudinger P. A. 1964; Oxidation of thiosulphate by intact cells of Thiobacillus X: effects of some experimental conditions. Australian Journal of Biological Science 17:738–751
    [Google Scholar]
  14. Villarejo M., Westley J. 1963; Rhodanese-catalysed reduction of thiosulphate by reduced lipoic acid. Journal of Biological Chemistry 238:1185–1186 pc
    [Google Scholar]
  15. Vishniac W., Trudinger P. A. 1962; Symposium on autotrophy. V. Carbon dioxide fixation and substrate oxidation in the chemosynthetic sulphur and hydrogen bacteria. Bacteriological Reviews 26:168–175
    [Google Scholar]
  16. Woolley D., Jones G. L., Happold F. C. 1962; Some metabolic differences between Thiobacillus thioparus, T. denitrificans, and T. thiocyanoxidans . Journal of General Microbiology 29:311–316
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-73-2-339
Loading
/content/journal/micro/10.1099/00221287-73-2-339
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error