SUMMARY: Biochemical and genetic evidence suggests that NADPH-dependent 2-keto-myo-inositol reductase and 2-keto-myo-inositol dehydratase are associated with myo-inositol catabolism in Salmonella typhimurium. When growth of this organism is initiated in salts medium containing citrate and inositol using a citrate-grown inoculum, ketoinositol reductase is virtually completely repressed. By contrast, when inositol-grown organisms are used as inoculum, a relatively high level of the enzyme is present in bacteria even after a 20-fold increase in mass in the citrate + inositol medium. Under these conditions, inositol is used for growth to a significant extent, and citrate is utilized to a lesser extent than when growth is started with citrate-grown organisms. Thus by varying the growth history of the organism, different biochemical phenotypes can be produced without any change in the genotype or in the immediate environmental conditions.
AndersonW. A.,
MagasanikB.1971a; The pathway of myo-inositol degradation in Aerobacter aerogenes. Identification of the intermediate, 2-deoxy-5-keto-d-gluconic acid. Journal of Biological Chemistry 246:5653–5661
AndersonW. A.,
MagasanikB.1971b; The pathway of myo-inositol degradation in Aerobacter aerogenes. Conversion of 2-deoxy-5-keto-d-gluconic acid to glycolytic intermediates. Journal of Biological Chemistry 246:5662–5675
BermanT.,
MagasanikB.1966a; The pathway of myoinositol degradation in Aerobacter aerogenes. Dehydrogenation and dehydration. Journal of Biological Chemistry 241:800–806
BermanT.,
MagasanikB.1966b; The pathway of myo-inositol degradation in Aerobacter aerogenes. Ring scission. Journal of Biological Chemistry 241:807–813
CharalampousF. C.1959; Biochemical studies on inositol. V. Purification and properties of the enzyme that cleaves inositol to d-glucuronic acid. Journal of Biological Chemistry 234:220–227
CohnM.1956; On the inhibition by glucose of the induced synthesis of β-galactosidase in Escherichia coli
. In Enzymes: Units of Biological Structure and Function, pp. 41–46 Edited by
GaeblerO. H.
New York: Academic Press;
DavisB. D.1956; Relations between enzymes and permeability (membrane transport) in bacteria. In Enzymes: Units of Biological Structure and Function, pp. 509–522 Edited by
GaeblerO. H.
New York: Academic Press;
LoewusF. A.,
KelleyS.,
NeufeldE. F.1962; Metabolism of myo-inositol in plants: conversion to pectin, hemicellulose, d-xylose and sugar acids. Proceedings of the National Academy of Sciences of the United States of America 48:421–425
LowryO. H.,
RosebroughN. J.,
FarrA. L.,
RandallR. J.1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
MonodJ.,
JacobF.1961; General conclusions: teleonomic mechanisms in cellular metabolism, growth and differentiation. Cold Spring Harbor Symposia on Quantitative Biology 26:389–401
SivakA.,
Hoffman-OstenhofO.1961; Enzymes of meso-inositol catabolism in the yeast, Schwanniomyces occidentalis
. Biochimica et biophysica acta 53:426–428