1887

Abstract

SUMMARY: Conidia of (Berk. & Curt.) M. B. Ellis strain c rapidly bound benzimidazole (BIZ), thiabendazole (TBZ), furidazole (FUD), and 2-phenyl benzimidazole (PBIZ) when mixed with aqueous solutions of the compounds at room temperature. Almost complete removal (> 95%) of the compounds from solution could be achieved by raising the spore/compound ratio in the reaction mixtures. The binding process was temperature-independent over the range of o to 65°C and pH-dependent (optimum at pH 4.5). BIZ binding was more sensitive to variation of ionic strength than was binding of TBZ, FUD or PBIZ.

Binding of BIZ conformed to the Langmuir adsorption isotherm; binding of the substituted benzimidazoles conformed to the isotherm over only part of the concentration range tested. Analysis of the binding data indicated that BIZ was bound to a single class of sites on the spore surface while the substituted benzimidazoles were bound to two or more classes differing in their affinity. This adsorption may be the first in a series of events leading to inhibition of spore germination by benzimidazole derivatives.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-73-1-85
1972-11-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/73/1/mic-73-1-85.html?itemId=/content/journal/micro/10.1099/00221287-73-1-85&mimeType=html&fmt=ahah

References

  1. Abramson H. A., Moyer L. W., Gorin M. H. 1942 Electrophoresis of Proteins and the Chemistry of Cell Surfaces. New York: Reinhold Publishing Co.;
    [Google Scholar]
  2. Bertaud W. S., Morice I. M., Russell D. W., Taylor A. 1963; The spore surface in Pithomyces chartarum . Journal of General Microbiology 32:385–395
    [Google Scholar]
  3. Bieber S., Hitchings G. H. 1955; The developing frog embryo and growth-inhibitors. Cancer Research Supplement 3:80–82
    [Google Scholar]
  4. Brown G. C. 1952; The influence of chemicals on the propagation of poliomyelitis virus in tissue culture. Journal of Immunology 69:441–450
    [Google Scholar]
  5. Campbell A. G. 1969; Prevention of facial eczema in grazing stock. New Zealand Journal of Agriculture 119:28–31
    [Google Scholar]
  6. Edgington L. V., Khew K. L., Barron G. L. 1971; Fungitoxic spectrum of benzimidazole compounds. Phytopathology 61:42–44
    [Google Scholar]
  7. Edsall J. T., Wyman J. 1958 Biophysical Chemistry vol. 1 p. 611 New York: Academic Press;
    [Google Scholar]
  8. Hayward D. O., Trapnell B. M. W. 1964 Chemisorption, 2nd edn. pp. 159–168 London: Butter- worths.;
    [Google Scholar]
  9. Horwitz W. 1970 Official Methods of Analysis of the Association of Official Analytical Chemists nth edn, pp. 750–751 Washington, D.C.: Association of Official Analytical Chemists;
    [Google Scholar]
  10. Langmuir I. 1916; The constitution and fundamental properties of solids and liquids. I. Solids. Journal of the American Chemical Society 38:2221–2295
    [Google Scholar]
  11. Lowry R. J., Sussman A. S. 1956; Physiology of the cell surface of Neurospora ascospores. II. Interference with dye adsorption by Polymyxin. Archives of Biochemistry and Biophysics 62:113–124
    [Google Scholar]
  12. McCalla T. M. 1940; Cation adsorption by bacteria. Journal of Bacteriology 40:23–32
    [Google Scholar]
  13. di Menna M. E., Campbell J., Mortimer P. H. 1970; Sporidesmin production and sporulation in Pithomyces chartarum . Journal of General Microbiology 61:87–96
    [Google Scholar]
  14. Rothstein A., Hayes A. D. 1956; The relationship of the cell surface to metabolism. XIII. The cationbinding properties of the yeast cell surface. Archives of Biochemistry and Biophysics 63:87–99
    [Google Scholar]
  15. Rudman D., Bixler T. J., Del Rio A. E. 1971; Effect of free fatty acids on binding of drugs by bovine serum albumin, by human serum albumin, and by rabbit serum. Journal of Pharmacology and Experimental Therapeutics 176:261–272
    [Google Scholar]
  16. Scatchard G. 1949; The attraction of proteins for small molecules and ions. Annals of the New York Academy of Science 51:660–672
    [Google Scholar]
  17. Sinclair D. P., Howe M. W. 1968; Effect of thiabendazole on Pithomyces chartarum (Berk. & Curt.) M. B. Ellis. New Zealand Journal of Agricultural Research 11. 59–62
  18. Slonimski P. P. 1956; Penetration du benzimidazole dans la cellule de levure. Experimental Cell Research 10:160–167
    [Google Scholar]
  19. Sussman A. S., von Boventer- Heidenhain B., Lowry R. J. 1957; Physiology of the cell surface of Neurospora ascospores. Plant Physiology 32:586–590
    [Google Scholar]
  20. Williams D. E., Coleman N. T. 1950; Cation exchange properties of plant root surfaces. Plant and Soil 2:243–256
    [Google Scholar]
  21. Woolley D. W. 1944; Some biological effects produced by benzimidazole and their reversal by purines. Journal of Biological Chemistry 152:225–232
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-73-1-85
Loading
/content/journal/micro/10.1099/00221287-73-1-85
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error