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SUMMARY: Verticillium albo-atrum required CO2 for growth when glucose or glycerol was the sole carbon source but not when succinate or acetate was supplied. The yeast form of the fungus fixed 14C from NaH14O3 into extracellular metabolites (I to 2 %), low-molecular-weight intracellular components (17 %), lipids (3 %), nucleic acids (34 %) and proteins (42 %). The only radioactive protein components were aspartate (33%), glutamate (33%), arginine+lysine (22%), and leucine + isoleucine (6 %). Nucleic acid bases that contained appreciable 14C were uracil (27 %), cytosine (21 %), adenine (22 %), guanine (27 %) and thymine (4 %). Carbon dioxide fixation appeared to be important for the anaplerotic biosynthesis of four-carbon intermediates and for purine biosynthesis.
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