1887

Abstract

SUMMARY

Walls from were partially lysed by an enzymatic complex present in filtrates from cultures of sp. When germinated spores were incubated with the enzymatic complex in a protective medium, osmotically sensitive sphaeroplasts were formed. The enzyme responsible for the degradation of the wall showed optimum activity at pH 7·5 and 32 °C. It was susceptible to the presence of divalent cations. The enzymatic complex released hexosamines (but no -acetyl hexosamines), neutral sugars and uronic acids from purified walls of the fungus. A wall-bound α-glucosidase was also rendered soluble by the enzyme. The enzymatic complex hydrolysed chitosan, but not chitin or mucoran, and hydrolysed the walls from only fungi belonging to the Mucorales. It is suggested that the hydrolytic capacity of filtrates from cultures of sp. developed in the presence of walls from was due to an enzyme (chitosanase), acting on chitosan but not on chitin.

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/content/journal/micro/10.1099/00221287-72-2-281
1972-09-01
2022-05-21
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