1887

Abstract

SUMMARY: Spore coat of ATCC6051 was fractionated as described by Kondo & Foster (1967). X-ray diffraction, infrared spectra, amino acid analyses and -terminal amino acid studies showed that most of the fractions resembled each other in physical and chemical structure and constituents. Electron micrographs of sections and shadowed replicas of the coat fractions at various stages showed that the inner part of the coat disappeared during treatment; no drastic morphological change was observed throughout the fractionation.

The amounts of cystine in the coat and the coat fractions were less than in keratins. This result and solubilizing experiments suggested that the spore coat does not chemically resemble keratins.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-72-1-87
1972-08-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/72/1/mic-72-1-87.html?itemId=/content/journal/micro/10.1099/00221287-72-1-87&mimeType=html&fmt=ahah

References

  1. ARONSON A. I., FITZ-JAMES P. C. 1968; Biosynthesis of bacterial spore coats.. Journal of Molecular Biology 33:199–212
    [Google Scholar]
  2. ASAJ M., TSUBOI M., SHIMANOUCHI T., MIZUSHIMA S. 1955; Infrared spectra of polypeptides and related compounds. I.. Journal of the American Chemical Society 59:322–325
    [Google Scholar]
  3. Crewther W. G., Fraser R. D. B., Lennox F. G., Lindley H. 1965; The chemistry of keratins.. In Advances in Protein Chemistry, 20:191–346
    [Google Scholar]
  4. ELLIOT A. 1954; The infra-red spectra of some optically active and meyo-synthetic polypeptides.. Proceedings of the Royal Society of London A221:104–114
    [Google Scholar]
  5. Elson L. A., Morgan W. T. J. 1933; A colorimetric method for the determination of glucosamine and chondrosamine.. Biochemical Journal 28:1824–1828
    [Google Scholar]
  6. GERHARDT P., Ribi E. 1964; Ultrastructure of the exosporium enveloping spores of Bacillus cereus.. Journal of Bacteriology 88:1774–1789
    [Google Scholar]
  7. Gould G. W., Hitchins A. D. 1963; Sensitization of bacterial spores to lysozyme and hydrogen peroxide with reagents which rupture disulphide bonds.. Nature, London 197:622
    [Google Scholar]
  8. GOULD G. W., HURST A. 1969 In The Bacterial Spore. (Frontispiece.). London:: Academic Press.;
    [Google Scholar]
  9. HOLT S. C., LEADBETTER E. R. 1969; Comparative ultrastructure of selected aerobic spore-forming bacteria: a freeze-etching study.. Bacteriological Reviews 33:346–378
    [Google Scholar]
  10. KADOTA H., IIJIMA K. 1965; The X-ray diffraction pattern of spores of Bacillus subtilis.. Agricultural and Biological Chemistry 29:80–81
    [Google Scholar]
  11. Kadota H., Iijima K., Uchida A. 1965; The presence of keratin-like substance in spore coat of Bacillus subtilis.. Agricultural and Biological Chemistry 29:870–875
    [Google Scholar]
  12. Kawasaki C., Nishihara T., Kondo M. 1969; Ultrastructure and its relation to the fractions isolated from spore coat of Bacillus megaterium.. Journal of Bacteriology 97:944–946
    [Google Scholar]
  13. Kondo M., Foster J. W. 1967; Chemical and electron microscope studies on fractions prepared from coats of bacillus spores.. Journal of General Microbiology 47:257–271
    [Google Scholar]
  14. MIYAZAWA T., BLOUT E. R. 1961; The infrared spectra of polypeptides in various conformations: amide I and II bands.. Journal of the American Chemical Society 83:712–719
    [Google Scholar]
  15. MURREL W. G. 1967 The biochemistry of the bacterial endospore.. In Advances in Microbial Physiology, vol 1pp. 133–251 Edited by Rose A. H., Wilkinson J. F. London:: Academic Press.;
    [Google Scholar]
  16. SACKS L. E., ALDERTON G. 1961; Behaviour of bacterial spores in aqueous polymer two-phase systems.. Journal of Bacteriology 82:331–341
    [Google Scholar]
  17. SALTON M. R. J., MARSHALL B. 1959; The composition of the spore wall and the wall of vegetative Cells of Bacillus subtilis.. Journal of General Microbiology 21:415–420
    [Google Scholar]
  18. Strange R. E., Dark F. A. 1956; The composition of the spore coats of Bacillus megaterium, B. subtilis and B. cereus.. Biochemical Journal 62:459–465
    [Google Scholar]
  19. VINTER V. 1959 Difference in cyst(e)ine content between vegetative cells and spore of Bacillus cereus and Bacillus megaterium.. Nature, London: 183998–999
    [Google Scholar]
  20. Vinter V. 1960; Spores of micro-organisms VIII. The synthesis of specific calcium and cystine-containing structures in sporulating cells of Bacilli.. Folia microbiologica 5:217–230
    [Google Scholar]
  21. VINTER V. 1961 The formation of cystine-rich structure in sporulating cells and its possible role in the resistance of spores.. In Spores, vol 11pp. 127–141 Edited by Halvorson H. O. >Minneapolis:: Burgess Publishing Co.;
    [Google Scholar]
  22. Warth A. D., OHYE D. F., Murell W. G. 1963; The compositions and structure of bacterial spores.. Journal of Cell Biology 16:579–592
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-72-1-87
Loading
/content/journal/micro/10.1099/00221287-72-1-87
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error