RT Journal Article SR Electronic(1) A1 PATON, W. H. N. A1 BUDD, K.YR 1972 T1 Zinc Uptake in Neocosmospora vasinfecta JF Microbiology, VO 72 IS 1 SP 173 OP 184 DO https://doi.org/10.1099/00221287-72-1-173 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Mycelium of Neocosmospora vasinfecta harvested in mid-logarithmic phase absorbs Zn2+ from dilute solutions in the absence of growth. Zn2+ uptake involves two phases: a rapidly established phase 1 believed to represent adsorption to negatively charged groups in the hyphal surface-membrane and a slowly established phase 2 which represents transport into the cytoplasm. Phase 1 is not influenced by low temperature, NaN3 or anaerobiosis applied for short periods, conforms to the Langmuir adsorption equation and is reduced in the presence of various other divalent cations. Phase 2 is strongly inhibited by low temperature, NaN3 and anaerobiosis and exhibits carrier-type kinetics with an apparent K m for Zn2+ of 0·2 mM. Mn2+ competitively inhibits phase 2 zinc uptake: Mg2+ acts as a ‘mixed-type’ inhibitor. Electron microscopy of unstained material indicates that part at least of phase 2 Zn is deposited in the cytoplasm and nucleus. A model for the metabolic uptake of Zn2+ involving phase 1 binding as a requisite preliminary process is suggested., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-72-1-173