1887

Microbiology Society logo

Volume 72, Issue 1

Fatty Acid Distribution in Triglycerides of Yeasts Grown on Glucose or -Alkanes Free

Abstract

SUMMARY

Lipid contents of yeasts grown on glucose were: 5·4%; 9·4%; 2·7%; 107, 41%; 12·5%; 2·7%; and 9·1%. In each yeast about 80% of the lipid consisted of triglycerides. When the triglycerides from five of the yeasts were analysed in detail, an unsaturated acid was invariably found at the 2-position. With 107 and about 50% of the total triglyceride fatty acids were saturated, resulting in over 50% of the triglycerides being of the 1,3-disaturated-2-monounsaturated type. When 107 and were grown on individual -alkanes, from C to C, the fatty-acid composition varied according to the chain length of the substrate, although with -tridecane neither yeast produced tridecanoic acid in the triglycerideand with -dodecane only contained an appreciable amount of dodecanoic acid in the triglyceride (32% of the fatty acids). With both yeasts on each alkane substrate, the lipid contents were not only lower than when grown on glucose but contained a smaller proportion of triglyceride. Saturated acids were now located at the 2-position of the triglycerides: 107 grown on -tetradecane produced 46% of its triglycerides with a saturated acid at the 2-position. The main advantage to be gained by growing yeasts on -alkanes is, as far as lipid formation is concerned, the biosynthesis of specific fatty acids rather than the production of plant-like triglycerides.

  • Accepted:
  • Published Online:
© Society for General Microbiology, 1972
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-72-1-151
1972-08-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/72/1/mic-72-1-151.html?itemId=/content/journal/micro/10.1099/00221287-72-1-151&mimeType=html&fmt=ahah

References

  1. BARRE C.B., DALLA M. S. J., PADLEY F. B. 1962; Separation of isomeric mixed triglycerides by thin layer chromatography using silica gel G impregnated with AgNO#.. Chemistry and Industry,1050–1051
     [Google Scholar]
  2. BARRON E. J., HANAHAN D. J. 1958; Observations on the silicic acid chromatography of the neutral lipids of rat liver, beef liver, and yeast.. Journal of Biological Chemistry 231:493–503
     [Google Scholar]
  3. BASS A., HOSPODKA J. 1952; Biosynthesis of fats by yeast. II. Composition of fat at various temperatures.. Chemicke Listy 46:243–245
     [Google Scholar]
  4. COLMAN M. H. 1961; Further studies on the pancreatic hydrolysis of some natural fats.. Journal of American Oil Chemists' Society 38:685–688
     [Google Scholar]
  5. COLMAN M. H. 1963; The structural investigation of natural fats.. Advances in Lipid Research 1:26–64
     [Google Scholar]
  6. COLMAN M. H., FULTON W. C. 1961 The structural investigation of natural fats by the partial hydrolysis technique.. In Enzymes of Lipid Metabolism pp 127–137 Edited by Desnuelle P. New York: Pergamon Press;
     [Google Scholar]
  7. DAVIS J. B. 1964; Microbial incorporation of fatty acids derived from n-alkanes into glycerides and waxes.. Applied Microbiology 12:210–214
     [Google Scholar]
  8. DESNUELLE P., SARI H., ENTRESSANGLES B. 1966; Positional specificity of pancreatic lipase.. Biochimica et biophysica acta 125:597–600
     [Google Scholar]
  9. FEDELI E., JACINI G. 1971; Lipid composition of vegetable oils.. Advances in Lipid Research 9:335–382
     [Google Scholar]
  10. FOLCH J., LEES M., SLOANE-STANLEY G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues.. Journal of Biological Chemistry 226:497–509
     [Google Scholar]
  11. HARRIES P. C., RATLEDGE C. 1969; Distribution of fatty acids in triglyceride from a yeast species grown on a fraction of n-alkanes predominant in tridecane.. Chemistry and Industry582–583
     [Google Scholar]
  12. HARTMAN L., HAWKE J. C., SHORLAND F. B., DIMENNA M. E. 1959; The fatty acid composition of Rhodotorula graminis fat.. Archives of Biochemistry and Biophysics 81:346–352
     [Google Scholar]
  13. HARVEY N. L., FEWSON C. A., HOLMS W. HXS. 1968; Apparatus for batch culture of microorganisms.. Laboratory Practice 17:1134–1136
     [Google Scholar]
  14. HOLMNERG J. 1948; Yeast lipids. 1. The component acids of Rhodotorula gracilis fat.. Svensk Kemisk Tidskrift 1:14–20
     [Google Scholar]
  15. HUNTER K., ROSE A. H. 1971 Yeast lipids and membranes.. In The Yeasts, vol. 211–270 Edited by Rose A. H., Harrison J. S. London: Academic Press;
     [Google Scholar]
  16. JOHNSON B., BROWN C. M. 1972; A possible relationship between the fatty acid composition of yeasts and the petite mutation.. Antonie van Leeuwenhoek (in the Press)
     [Google Scholar]
  17. JOHNSON B., NELSON S. J., BROWN C. M. 1972; Influence of glucose concentration on the physiology and lipid composition of some yeasts.. Antonie van Leeuwenhoek (in the Press)
     [Google Scholar]
  18. JURRIENS G. 1968 Analysis of glycerides. In Analysis and Characterisation of Oils, Fats and Fat Products. vol 11 pp. 217–298 Edited by Boekenoogen H. A. London: Interscience;
     [Google Scholar]
  19. KESSELL R. H. J. 1968; Fatty acid of Rhodotorular gracilis: fat production in submerged culture and the particular effect of pH value.. Journal of Applied Bacteriology 31:220–231
     [Google Scholar]
  20. LUDVIK J., MUNK V., DOSTALEK M. 1968; Ultrastructural changes in the yeast Candida lipolytica caused by penetration of hydrocarbons into the cell.. Experientia 24:1066–1068
     [Google Scholar]
  21. MARINETTI G. 1962; Chromatographic separation, identification and analysis of phosphatides.. Journal of Lipid Research 3:1–20
     [Google Scholar]
  22. NICHOLS B. W., JAMES A. T. 1964; The lipids of plant storage tissue.. Fette, Seifen, Anstrichmittel 66:1003–1006
     [Google Scholar]
  23. RATLEDGE C. 1968; Production of fatty acids and lipid by a Candida sp. growing on a fraction of ra-alkanes predominating in tridecane.. Biotechnology and Bioengineering 10:511–533
     [Google Scholar]
  24. RATLEDGE C. 1970; Microbial conversions of n-alkanes to fatty acids: a new attempt to obtain economical microbial fats and fatty acids.. Chemistry and Industry843–854
     [Google Scholar]
  25. RATLEDGE C. 1971; The industrial potential of microbial lipids.. Journal of General Microbiology 68:xxiii
     [Google Scholar]
  26. SHAW R. 1966; The polyunsaturated fatty acids of microorganisms.. Advances in Lipid Research 4:107–174
     [Google Scholar]
  27. STERN I., SHAPIRO B. 1953; A rapid and simple method for the determination of esterified fatty acids and for total fatty acids in blood.. Journal of Clinical Pathology 6:158–160
     [Google Scholar]
  28. THOMAS A. E., SCHAROUN J. E., RALSTON H. 1965; Quantitative estimation of isomeric monoglycerides by thin-layer chromatography.. Journal of American Oil Chemists' Society 42:789–792
     [Google Scholar]
  29. VANDER WAL R. J. 1960; Calculation of the distribution of the saturated and unsaturated acyl groups in fats, from pancreatic lipase hydrolysis data.. Journal of American Oil Chemists' Society 37:18–20
     [Google Scholar]
  30. VANDER WAL R. J. 1964; Triglyceride structure.. Advances in Lipid Research 2:1–16
     [Google Scholar]
  31. WILLS E. D. 1965; Lipases.. Advances in Lipid Research 3:197–240
     [Google Scholar]
  32. WOODBINE M. 1959 Microbial fat: microorganisms as potential fat producers.. In Progress in Industrial Microbiology vol 1 pp. 181–245 Edited by Hockenhull D. J. D. London: HeywoodCo;
     [Google Scholar]
/content/journal/micro/10.1099/00221287-72-1-151
Loading
Fatty Acid Distribution in Triglycerides of Yeasts Grown on Glucose or n-Alkanes
Microbiology 72, 151 (1972); https://doi.org/10.1099/00221287-72-1-151
/content/journal/micro/10.1099/00221287-72-1-151
/content/journal/micro/10.1099/00221287-72-1-151
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error