@article{mbs:/content/journal/micro/10.1099/00221287-72-1-143, author = "RATLEDGE, C. and HALL, M. J.", title = "Isolation and Properties of Auxotrophic Mutants of Mycobacterium smegmatis Requiring either Salicylic Acid or Mycobactin", journal= "Microbiology", year = "1972", volume = "72", number = "1", pages = "143-150", doi = "https://doi.org/10.1099/00221287-72-1-143", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-72-1-143", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY A mutant of Mycobacterium smegmatis required 3 to 4 μg salicylate, or certain other phenolic acids/ml to restore optimal growth. Iron-deficient growth did not increase the requirement for salicylate. Salicylate, but nota salicylate-substitute was incorporated solely into mycobactin S. Mycobactin S was absorbed by growing bacteria but it did not substitute or spare the requirement for salicylate. Another mutant has been produced which required about 0·2 μg desferrimycobactin S/ml to restore growth under iron-deficient conditions. Salicylate, citrate, cobactin S with mycobactic acid S or Tween 80 did not substitute for mycobactin, but iron at 2 or more μg/ml permitted growth without mycobactin. Under these growth conditions the mycobactin requirement would be minute and some synthesis or carry-over during inoculation cannot be eliminated as a source of mycobactin. Salicylate and mycobactin were thus both required for growth.", }