1887

Abstract

SUMMARY: Small numbers of several bacterial species were rapidly and specifically detected with the I-labelled antibody method described previously. Multibacterial species detection was achieved in one operation with I-labelled mixtures of type-specific anti-bacterial globulins but the level and variability of the blank value increased, and sensitivity decreased, with the number of globulin components in the mixture. An indirect radio-assay that involved successive treatments of bacteria with unlabelled rabbit anti-bacterial serum and I-labelled immunopurified goat anti-rabbit globulin was less sensitive and reproducible than the direct radio-assay. A modified assay, developed to detect and determine bacteria filtered on to a membrane filter, allowed the detection of small numbers of bacteria in large volumes of aqueous sample.

The feasibility of rapidly and specifically detecting small numbers of virus particles with I-labelled anti-viral globulin was investigated with bacteriophage T7 as a model particle. Methods for the rapid separation of phage-I-labelled globulin complex were developed and a minimum of about 5–10 total phage particles was detected.

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/content/journal/micro/10.1099/00221287-72-1-127
1972-08-01
2022-01-24
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References

  1. ADAMS M. H. 1959 Bacteriophages. pp 27–34. New York: Interscience Publishers, Inc.;
    [Google Scholar]
  2. ASHCROFT J. 1970; Gamma counting of iodine125 using a metal-loaded liquid scintillator.. Analytical Biochemistry 37:268–275
    [Google Scholar]
  3. AVRAMEAS S., TERNYNCK T. 1969; The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.. Immunochemistry 6:53–66
    [Google Scholar]
  4. CLOSS O. 1968; Rapid identification of small numbers of bacteria after short time cultivation in the presence of FITC-conjugated antiserum.. Acta pathologica microbiologica scandinavica 72:412–420
    [Google Scholar]
  5. COOMBS R. R. A., MOURANT A. E., RACE R. R. 1945; A new test for the detection of weak and ‘incomplete’ Rh agglutinins.. British Journal of Experimental Pathology 26:255–266
    [Google Scholar]
  6. DANIELSSON D. 1965; A membrane filter method for the demonstration of bacteria by the fluorescent antibody technique. I.A methodological study.. Acta pathologica microbiologica scandinavica 63:597–603
    [Google Scholar]
  7. DANIELSSON D., LAURELL G. 1965; A membrane filter method for the demonstration of bacteria by the fluorescent antibody technique. 2. The application of the method for detection of small numbers of bacteria in water.. Acta pathologica microbiologica scandinavica 63:604–608
    [Google Scholar]
  8. GLOVER J. S., SALTER D. N., SHEPHERD B. P. 1967; A study of some factors that influence the iodination of ox insulin.. Biochemical Journal 103:120–128
    [Google Scholar]
  9. GUTHRIE R. K., REEDER D. J. 1969; Membrane filter-fluorescent-antibody method for detection and enumeration of bacteria in water.. Applied Microbiology 17:399–401
    [Google Scholar]
  10. HUNTER W. M., GREENWOOD F. C. 1962 Preparation of iodine-131 labelled human growth hormone of high specific activity.. Nature London : 194495–496
    [Google Scholar]
  11. ISENBERG H. 1963 Biochemistry of bacteriophage μ2.. M.Sc.Thesis University of Birmingham:
    [Google Scholar]
  12. JOST R., FEY H. 1971; Rapid detection of small numbers of airborne bacteria by a membrane filter fluorescent-antibody technique.. Applied Microbiology 20:861–863
    [Google Scholar]
  13. KABAT E. A., MAYER M. M. 1961 Experimental Immunochemistry.. Springfield Illinois: Charles C. Thomas.;
    [Google Scholar]
  14. KENT L. H., SLADE J. H. R. 1960; Immunochemically-active cross-linked polystyrene preparations.. Biochemical Journal 77:12–17
    [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin. phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. MCFARLANE A. S. 1956; Labelling of plasma proteins with radioactive iodine.. Biochemical Journal 62:135–143
    [Google Scholar]
  17. MCFARLANE A. S. 1958; Efficient trace-labelling of proteins with iodine.. Nature London : 182:53.
    [Google Scholar]
  18. SACKS L. E., ALDERTON G. 1961; Behaviour of bacterial spores in aqueous polymer-two-phase systems.. Journal of Bacteriology 82:331–341
    [Google Scholar]
  19. SHARER J. M., KLEIN F., LINCOLN R. E. 1968; Growth and metabolism of live vaccine strain of Pasteurella tularensis.. Applied Microbiology 16:855–861
    [Google Scholar]
  20. STEPHEN J., GALLOP R. G. C., SMITH H. 1966; Separation of antigens by immunological specificity.. Biochemical Journal 101:717–720
    [Google Scholar]
  21. STRANGE R. E., POWELL E. O., PEARCE T. W. 1971; The rapid detection and determination of sparse bacterial populations with radioactively-labelled homologous antibodies.. Journal of General Microbiology 67:349–357
    [Google Scholar]
  22. TIGERTT W. D. 1962; Soviet viable Pasteurella tularensis vaccines.A review of selected articles.. Bacteriological Reviews 26:354–372
    [Google Scholar]
  23. WARBURG O., CHRISTIAN W. 1942; Isolation and crystallization of enolase.. Biochemische Zeitschriff 310:384–421
    [Google Scholar]
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