Summary: Out of nine strains of from culture collections, only five grew in a minimal medium that contained glucose and ammonium sulphate as principal nutrients. One strain required biotin, while three other strains required two or more amino acids for growth. A freshly isolated strain of required three amino acids for growth.

Three strains of from a culture collection each required two or more amino acids for growth. From two of these strains, substrains independent of amino acids were developed by serial transfers. Ability to grow in a minimal medium is therefore not so valuable as a distinction between and as previously had been supposed.


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